Spontaneous and UV-induced mutations in Escherichia coli K-12 strains with altered or absent DNA polymerase I - PubMed (original) (raw)
Spontaneous and UV-induced mutations in Escherichia coli K-12 strains with altered or absent DNA polymerase I
H Bates et al. J Bacteriol. 1989 May.
Abstract
The induction of mutations to valine resistance and to rifampin resistance occurs after UV irradiation in bacteria carrying a deletion through the polA gene (delta polA), showing that DNA polymerase I (PolI) is not an essential enzyme for this process. The PolI deletion strain showed a 7- to 10-fold-higher spontaneous mutation frequency than the wild type. The presence in the deletion strain of the 5'----3' exonuclease fragment on an F' episome caused an additional 10-fold increase in spontaneous mutation frequency, resulting in mutation frequencies on the order of 50- to 100-fold greater than wild type. The mutator effect associated with the 5'----3' exonuclease gene fragment together with much of the effect attributable to the polA deletion was blocked in bacteria carrying a umuC mutation. The mutator activity therefore appears to reflect constitutive SOS induction. Excision-proficient polA deletion strains exhibited increased sensitivity to the lethal effect of UV light which was only partially ameliorated by the presence of polA+ on an F' episome. The UV-induced mutation rate to rifampin resistance was marginally lower in delta polA bacteria than in bacteria carrying the polA+ allele. This effect is unlikely to be caused by the existence of a PolI-dependent mutagenic pathway and is probably an indirect effect caused by an alteration in the pattern of excision repair, since it did not occur in excision-deficient (uvrA) bacteria. An excision-deficient polA deletion strain possessed UV sensitivity similar to that of an isogenic strain carrying polA+ on an F' episome, showing that none of the functions of PolI are needed for postreplication repair in the absence of excision repair. Our data provide no evidence for a pathway of UV mutagenesis dependent on PolI, although it remains an open question whether PolI is able to participate when it is present.
Similar articles
- Role of DNA polymerase I in postreplication repair: a reexamination with Escherichia coli delta polA.
Sharma RC, Smith KC. Sharma RC, et al. J Bacteriol. 1987 Oct;169(10):4559-64. doi: 10.1128/jb.169.10.4559-4564.1987. J Bacteriol. 1987. PMID: 3308845 Free PMC article. - Escherichia coli mutator (Delta)polA is defective in base mismatch correction: the nature of in vivo DNA replication errors.
Tago Y, Imai M, Ihara M, Atofuji H, Nagata Y, Yamamoto K. Tago Y, et al. J Mol Biol. 2005 Aug 12;351(2):299-308. doi: 10.1016/j.jmb.2005.06.014. J Mol Biol. 2005. PMID: 16005896 - Involvement of Escherichia coli K-12 DNA polymerase I in the growth of bacteriophage Mu.
McBeth DL, Taylor AL. McBeth DL, et al. J Virol. 1983 Oct;48(1):149-56. doi: 10.1128/JVI.48.1.149-156.1983. J Virol. 1983. PMID: 6224939 Free PMC article. - Current understanding of UV-induced base pair substitution mutation in E. coli with particular reference to the DNA polymerase III complex.
Bridges BA, Woodgate R, Ruiz-Rubio M, Sharif F, Sedgwick SG, Hübscher U. Bridges BA, et al. Mutat Res. 1987 Dec;181(2):219-26. doi: 10.1016/0027-5107(87)90099-6. Mutat Res. 1987. PMID: 3317025 Review. - Genetic control of radiation sensitivity and DNA repair in Neurospora.
Schroeder A. Schroeder A. Basic Life Sci. 1975;5B:567-76. doi: 10.1007/978-1-4684-2898-8_22. Basic Life Sci. 1975. PMID: 1103873 Review.
Cited by
- Adaptive mutation in Escherichia coli.
Foster PL. Foster PL. Cold Spring Harb Symp Quant Biol. 2000;65:21-9. doi: 10.1101/sqb.2000.65.21. Cold Spring Harb Symp Quant Biol. 2000. PMID: 12760017 Free PMC article. Review. No abstract available. - Activity of the purified mutagenesis proteins UmuC, UmuD', and RecA in replicative bypass of an abasic DNA lesion by DNA polymerase III.
Rajagopalan M, Lu C, Woodgate R, O'Donnell M, Goodman MF, Echols H. Rajagopalan M, et al. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10777-81. doi: 10.1073/pnas.89.22.10777. Proc Natl Acad Sci U S A. 1992. PMID: 1438275 Free PMC article. - Identification, sequencing, and targeted mutagenesis of a DNA polymerase gene required for the extreme radioresistance of Deinococcus radiodurans.
Gutman PD, Fuchs P, Ouyang L, Minton KW. Gutman PD, et al. J Bacteriol. 1993 Jun;175(11):3581-90. doi: 10.1128/jb.175.11.3581-3590.1993. J Bacteriol. 1993. PMID: 8501062 Free PMC article. - Processing closely spaced lesions during Nucleotide Excision Repair triggers mutagenesis in E. coli.
Janel-Bintz R, Napolitano RL, Isogawa A, Fujii S, Fuchs RP. Janel-Bintz R, et al. PLoS Genet. 2017 Jul 7;13(7):e1006881. doi: 10.1371/journal.pgen.1006881. eCollection 2017 Jul. PLoS Genet. 2017. PMID: 28686598 Free PMC article. - Interactions between p53, hMSH2-hMSH6 and HMG I(Y) on Holliday junctions and bulged bases.
Subramanian D, Griffith JD. Subramanian D, et al. Nucleic Acids Res. 2002 Jun 1;30(11):2427-34. doi: 10.1093/nar/30.11.2427. Nucleic Acids Res. 2002. PMID: 12034830 Free PMC article.
References
- Mutat Res. 1969 Nov-Dec;8(3):431-9 - PubMed
- J Biol Chem. 1988 Dec 15;263(35):18946-52 - PubMed
- J Mol Biol. 1971 Jun 14;58(2):623-30 - PubMed
- Genetics. 1970 Oct;66(2):187-217 - PubMed
- Mutat Res. 1972 Sep;16(7):13-25 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources