Individual protein-binding domains of the insulin gene enhancer positively activate beta-cell-specific transcription - PubMed (original) (raw)
Individual protein-binding domains of the insulin gene enhancer positively activate beta-cell-specific transcription
O Karlsson et al. Mol Cell Biol. 1989 Feb.
Abstract
A beta-cell-specific enhancer is located in the 5'-flanking DNA of the rat insulin 1 gene. Two homologous 8-base-pair sequences in the enhancer (IEB1 and IEB2) significantly stimulated transcription from a heterologous promoter (two- to fourfold) in a cell-specific fashion. When the elements were combined or duplicated, more than 50% of the activity of the intact enhancer was obtained. These two cis-acting elements appear to play a dominant role in the positive control of beta-cell-specific transcription of the insulin gene.
Similar articles
- Regulatory regions of rat insulin I gene necessary for expression in transgenic mice.
Dandoy-Dron F, Monthioux E, Jami J, Bucchini D. Dandoy-Dron F, et al. Nucleic Acids Res. 1991 Sep 25;19(18):4925-30. doi: 10.1093/nar/19.18.4925. Nucleic Acids Res. 1991. PMID: 1923760 Free PMC article. - A beta-cell-specific protein binds to the two major regulatory sequences of the insulin gene enhancer.
Ohlsson H, Karlsson O, Edlund T. Ohlsson H, et al. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4228-31. doi: 10.1073/pnas.85.12.4228. Proc Natl Acad Sci U S A. 1988. PMID: 3288987 Free PMC article. - Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5' flanking elements.
Edlund T, Walker MD, Barr PJ, Rutter WJ. Edlund T, et al. Science. 1985 Nov 22;230(4728):912-6. doi: 10.1126/science.3904002. Science. 1985. PMID: 3904002 - Insulin-producing cells contain a cell-specific repressor activity that functions through multiple E-box sequences.
Leshkowitz D, Aronheim A, Walker MD. Leshkowitz D, et al. DNA Cell Biol. 1992 Sep;11(7):549-58. doi: 10.1089/dna.1992.11.549. DNA Cell Biol. 1992. PMID: 1388727 - [Transcription factors for the insulin gene].
Kajimoto Y, Kawamori R, Watada H, Kamada T. Kajimoto Y, et al. Nihon Rinsho. 1994 Oct;52(10):2535-43. Nihon Rinsho. 1994. PMID: 7983774 Review. Japanese.
Cited by
- The insulin and islet amyloid polypeptide genes contain similar cell-specific promoter elements that bind identical beta-cell nuclear complexes.
German MS, Moss LG, Wang J, Rutter WJ. German MS, et al. Mol Cell Biol. 1992 Apr;12(4):1777-88. doi: 10.1128/mcb.12.4.1777-1788.1992. Mol Cell Biol. 1992. PMID: 1549125 Free PMC article. - The helix-loop-helix transcription factor USF (upstream stimulating factor) binds to a regulatory sequence of the human insulin gene enhancer.
Read ML, Clark AR, Docherty K. Read ML, et al. Biochem J. 1993 Oct 1;295 ( Pt 1)(Pt 1):233-7. doi: 10.1042/bj2950233. Biochem J. 1993. PMID: 8216223 Free PMC article. - c-jun inhibits insulin control element-mediated transcription by affecting the transactivation potential of the E2A gene products.
Robinson GL, Henderson E, Massari ME, Murre C, Stein R. Robinson GL, et al. Mol Cell Biol. 1995 Mar;15(3):1398-404. doi: 10.1128/MCB.15.3.1398. Mol Cell Biol. 1995. PMID: 7862133 Free PMC article. - Nuclear protein-binding sites in a transcriptional control region of the rabbit alpha-globin gene.
Yost SE, Shewchuk B, Hardison R. Yost SE, et al. Mol Cell Biol. 1993 Sep;13(9):5439-49. doi: 10.1128/mcb.13.9.5439-5449.1993. Mol Cell Biol. 1993. PMID: 8355692 Free PMC article. - The homeodomain protein IPF-1/STF-1 is expressed in a subset of islet cells and promotes rat insulin 1 gene expression dependent on an intact E1 helix-loop-helix factor binding site.
Serup P, Petersen HV, Pedersen EE, Edlund H, Leonard J, Petersen JS, Larsson LI, Madsen OD. Serup P, et al. Biochem J. 1995 Sep 15;310 ( Pt 3)(Pt 3):997-1003. doi: 10.1042/bj3100997. Biochem J. 1995. PMID: 7575438 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1981 Jul;78(7):4339-43 - PubMed
- Mol Cell Biol. 1988 Jun;8(6):2620-7 - PubMed
- Proc Natl Acad Sci U S A. 1982 Nov;79(21):6453-7 - PubMed
- Mol Cell Biol. 1982 Sep;2(9):1044-51 - PubMed
- Nature. 1983 Dec 8-14;306(5943):557-61 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical