A whole new ball game: Stem cell-derived epithelia in the study of host-microbe interactions - PubMed (original) (raw)

Review

A whole new ball game: Stem cell-derived epithelia in the study of host-microbe interactions

Jhansi L Leslie et al. Anaerobe. 2016 Feb.

Abstract

Recent advances in developmental and stem cell biology have resulted in techniques that enable the generation and maintenance of complex epithelium in vitro. While these models have been utilized to study host development and disease, a renewed appreciation of host-microbe interactions has sparked interest in employing these new techniques to study microbes at the epithelial interface. Here we review the current advances in host-microbe interactions that have resulted from experiments using these complex epithelia. Furthermore we highlight aspects of these techniques that warrant further development to facilitate the study of host-microbe interactions.

Keywords: Clostridium difficile; Enteroid; Helicobacter pylori; Organoid; Salmonella enterica.

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Figures

Figure 1. Using enteroids and organoids, epithelial surrogates of gastrointestinal organs, to study host–microbe interactions

Clockwise from the right, enteroids derived from the stomach were utilized to examine how H. pylori responds to urea (18). Colonic organoids have been shown to be sensitive to treatment with C. difficile toxin (20). Organoids that resemble the ileum have been used to study S. enterica Serovar Typhi and C. difficile (11, 23). Gallbladder derived enteroids were used to investigate the association between infection with S. enterica Serovar Typhi and development of gallbladder cancer (12).

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A whole new ball game: Stem cell-derived epithelia in the study of host-microbe interactions - PubMed (original) (raw)