IL-6/STAT3/SOCS3 signaling pathway playing a regulatory role in ulcerative colitis carcinogenesis - PubMed (original) (raw)
. 2015 Aug 15;8(8):12009-17.
eCollection 2015.
Zhi-Bin Ma 1, Hong-Yu Xu 1, Li-Jun Shi 1, Dong-Yue Li 1, Li-Ying Sun 1, Xun-Hai Yin 1, Guo-Yin Sang 1, Dan Xu 1, Yin-Hua Tang 1, Xi Wang 1, Peng Li 1, Feng Wu 1, Jin Zhou 2
Affiliations
- PMID: 26550113
- PMCID: PMC4612798
IL-6/STAT3/SOCS3 signaling pathway playing a regulatory role in ulcerative colitis carcinogenesis
Ying-Ying Chen et al. Int J Clin Exp Med. 2015.
Abstract
Objective: Large-scale clinical studies have shown that ulcerative colities were related with colorectal cancer. In this study, animal model was established by AOM/DSS method to explore the activation of IL-6-STAT3-SOCS3 signaling pathway and the expression of pathway-related proteins in ulcerative colitis carcinogenesis, in order to lay a foundation for exploring the regulation mechanism of IL-6/STAT3/SOCS3 signaling pathway in ulcerative colitis carcinogenesis.
Method: AOM/DSS modeling method was used to establish animal models of ulcerative colitis carcinogenesis; colonic mucosa specimens were collected at different time points for pathological examination. Immunohistochemical method and western blot were used to detect the expression of IL6, STAT3 and SOCS3 protein in the control group, UC model + empty vector group and UC model + STAT3 knockout group.
Results: In UC model + empty vector group, IL6 and STAT3 expression was increased as lesion degree increased (P < 0.05). The expression of SOCS3 was weakened and the degree of activation decreased (P < 0.05). IL6 expression increased in UC model + STAT3 knockout group (P < 0.05) while the expression of SOCS3 decreased; compared with the UC model + empty vector group, there was a significant difference (P < 0.05).
Conclusion: The expression and activation of IL6 and STAT3 expression were enhanced in ulcerative colitis carcinogenesis, and their expression increased with the lesion degree increased, reflecting the disease progression to a certain extent. The expression and activation of SOCS3 expression decreased. STAT3 had a certain effect on the expression of SOCS3, playing a certain regulatory role in ulcerative colitis carcinogenesis.
Keywords: IL-6; SOCS3; STAT3; Ulcerative colitis; signaling pathways.
Figures
Figure 1
The image of pathological inflammation in mice. A: Normal mucosa; B-D: Mucosal pathological images at different times.
Figure 2
Inflammation-dysplasia-cancer carcinogenesis pathological process. A: Mild dysplasia; B: The severe dysplasia; C: Invasive cancer.
Figure 3
IL-6 expression level in different groups at different stages.
Figure 4
SATA3 expression levels in different groups at different stages.
Figure 5
SOCS3 expression levels in different groups at different stages.
Figure 6
IL-6 protein expression in different time points in each group.
Figure 7
IL-6 protein expression level in different group at different stages.
Figure 8
IL-6 protein expression in different time points in each group.
Figure 9
SATA3 protein expression level in different group at different stages.
Figure 10
IL-6 protein expression in different time points in each group.
Figure 11
SATA3 protein expression level in different group at different stages.
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