Gene activation in somatic nuclei after injection into amphibian oocytes - PubMed (original) (raw)

Gene activation in somatic nuclei after injection into amphibian oocytes

E M De Robertis et al. Proc Natl Acad Sci U S A. 1977 Jun.

Abstract

Genes that are unexpressed in somatic cells have been activated by injecting cultured cell nuclei of the frog Xenopus laevis into oocytes of the newt Pleurodeles waltlii. The genes that were activated are normally expressed in oocytes but not in cultured cells. Conversely, genes that are normally expressed in cultured cells but not in oocytes became inactive when cultured cell nuclei were injected into oocytes. These changes in gene activity were detected by two-dimensional gel electrophoresis of proteins synthesized by oocytes injected with nuclei. Controls, which included the use of alpha-amanitin, showed that these changes in protein synthesis are dependent on gene transcription. We conclude that genes that become inactive during cell differentiation can be reactivated, in the absence of cell division, by normal components of oocyte cytoplasm.

PubMed Disclaimer

Similar articles

• Probing the program of gene expression utilized in early development.
  De Robertis EM. De Robertis EM. Arch Biol Med Exp. 1979 Oct;12(3):325-9. Arch Biol Med Exp. 1979. PMID: 95388
• Injected amphibian oocytes: a living test tube for the study of eukaryotic gene transcription?
  de Robertis EM, Gurdon JB, Partington GA, Mertz JE, Laskey RA. de Robertis EM, et al. Biochem Soc Symp. 1977;(42):181-91. Biochem Soc Symp. 1977. PMID: 339919 Review.
• The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
  Korn LJ, Gurdon JB. Korn LJ, et al. Nature. 1981 Feb 5;289(5797):461-5. doi: 10.1038/289461a0. Nature. 1981. PMID: 7193290
• Somatic nuclei in amphibian oocytes: evidence for selective gene expression.
  De Robertis EM, Partington GA, Longthorne RF, Gurdon JB. De Robertis EM, et al. J Embryol Exp Morphol. 1977 Aug;40:199-214. J Embryol Exp Morphol. 1977. PMID: 562385
• Reprogramming of transplanted nuclei in amphibia.
  Gurdon JB, Laskey RA, De Robertis EM, Partington GA. Gurdon JB, et al. Int Rev Cytol Suppl. 1979;(9):161-78. doi: 10.1016/s0074-7696(08)60902-x. Int Rev Cytol Suppl. 1979. PMID: 385535 Review. No abstract available.

Cited by

• The role of autophagy in morphogenesis and stem cell maintenance.
  Offei EB, Yang X, Brand-Saberi B. Offei EB, et al. Histochem Cell Biol. 2018 Dec;150(6):721-732. doi: 10.1007/s00418-018-1751-0. Epub 2018 Oct 31. Histochem Cell Biol. 2018. PMID: 30382373 Review.
• Factors and molecules that could impact cell differentiation in the embryo generated by nuclear transfer.
  Simões R, Rodrigues Santos A Jr. Simões R, et al. Organogenesis. 2017 Oct 2;13(4):156-178. doi: 10.1080/15476278.2017.1389367. Organogenesis. 2017. PMID: 29020571 Free PMC article. Review.
• The Egg and the Nucleus: A Battle for Supremacy.
  Gurdon JB. Gurdon JB. Rambam Maimonides Med J. 2015 Jul 30;6(3):e0023. doi: 10.5041/RMMJ.10208. Rambam Maimonides Med J. 2015. PMID: 26241222 Free PMC article.
• Lessons from a great developmental biologist.
  De Robertis EM. De Robertis EM. Differentiation. 2014 Jul;88(1):3-8. doi: 10.1016/j.diff.2013.12.004. Epub 2014 Nov 20. Differentiation. 2014. PMID: 25455202 Free PMC article.
• Profile of Edward M. De Robertis.
  Viegas J. Viegas J. Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20349-51. doi: 10.1073/pnas.1320552110. Epub 2013 Nov 25. Proc Natl Acad Sci U S A. 2013. PMID: 24277844 Free PMC article. No abstract available.

References

1. 1. Biochem J. 1963 Oct;89:114-23 - PubMed
2. 1. Dev Biol. 1976 Sep;52(2):201-9 - PubMed
3. 1. J Embryol Exp Morphol. 1976 Dec;36(3):523-40 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1972 Mar;69(3):571-5 - PubMed
5. 1. Nature. 1970 Jan 10;225(5228):184-7 - PubMed

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central