Higher-order structure of Saccharomyces cerevisiae chromatin - PubMed (original) (raw)

Comparative Study

Higher-order structure of Saccharomyces cerevisiae chromatin

P T Lowary et al. Proc Natl Acad Sci U S A. 1989 Nov.

Abstract

We have developed a method for partially purifying chromatin from Saccharomyces cerevisiae (baker's yeast) to a level suitable for studies of its higher-order folding. This has required the use of yeast strains that are free of the ubiquitous yeast "killer" virus. Results from dynamic light scattering, electron microscopy, and x-ray diffraction show that the yeast chromatin undergoes a cation-dependent folding into 30-nm filaments that resemble those characteristic of higher-cell chromatin; moreover, the packing of nucleosomes within the yeast 30-nm filaments is similar to that of higher cells. These results imply that yeast has a protein or protein domain that serves the role of the histone H 1 found in higher cells; physical and genetic studies of the yeast activity could help elucidate the structure and function of H 1. Images of the yeast 30-nm filaments can be used to test crossed-linker models for 30-nm filament structure.

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References

1. 1. EMBO J. 1983;2(8):1367-72 - PubMed
2. 1. J Cell Biol. 1982 Apr;93(1):217-22 - PubMed
3. 1. J Cell Biol. 1984 Apr;98(4):1320-7 - PubMed
4. 1. Nature. 1984 Oct 11-17;311(5986):532-7 - PubMed
5. 1. Nucleic Acids Res. 1984 Nov 12;12(21):7975-85 - PubMed

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