Duplication mutation as an SOS response in Escherichia coli: enhanced duplication formation by a constitutively activated RecA - PubMed (original) (raw)

Duplication mutation as an SOS response in Escherichia coli: enhanced duplication formation by a constitutively activated RecA

J Dimpfl et al. Genetics. 1989 Oct.

Abstract

The SOS response in Escherichia coli involves the induction of a multioperon regulatory system, which copes with the presence of DNA lesions that interfere with DNA replication. Induction depends on activation of the RecA protein to cleave the LexA repressor of SOS operons. In addition to inducible DNA repair, the SOS system produces a large increase in the frequency of point mutations. To examine the possibility that other types of mutations are induced as part of the SOS response, we have studied the production of tandem duplications. To avoid the complications of indirect effects of the DNA lesions, we have activated the SOS response by a constitutive mutation in the recA gene, recA730. The introduction of the recA730 mutation results in an increase in duplications in the range of tenfold or greater, as judged by two different criteria. Based on its genetic requirements, the pathway for induced duplication formation is distinct from the point mutation pathway and also differs from the major normal recombination pathway. The induction of pathways for both duplications and point mutations shows that the SOS system produces a broad mutagenic response. We have suggested previously that many types of mutations might be induced by severe environmental stress, thereby enhancing genetic variation in an endangered population.

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Duplication mutation as an SOS response in Escherichia coli: enhanced duplication formation by a constitutively activated RecA - PubMed (original) (raw)