DNA replication origin and transcriptional enhancer in c-myc gene share the c-myc protein binding sequences - PubMed (original) (raw)

DNA replication origin and transcriptional enhancer in c-myc gene share the c-myc protein binding sequences

H Ariga et al. EMBO J. 1989.

Abstract

We have previously reported that c-myc protein, or protein(s) complexed with c-myc protein, binds to the region upstream of the first exon of the c-myc gene and that this region contains an origin of cellular DNA replication (ori) and also a transcriptional enhancer. Here we show by Southwestern blotting that c-myc protein binds directly to a 7 bp sequence within the above region. Furthermore, we show that the c-myc protein binding sequences are indispensable for both ori and enhancer functions, but that additional sequences are required for maximal ori and enhancer activities. Thus, c-myc protein is a sequence specific factor which is apparently used both in initiation of DNA replication and in regulation of RNA transcription.

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References

1. 1. EMBO J. 1989 Apr;8(4):1111-9 - PubMed
2. 1. Nature. 1987 Oct 1-7;329(6138):456-8 - PubMed
3. 1. Oncogene. 1988 Nov;3(5):509-15 - PubMed
4. 1. J Mol Biol. 1967 Jun 14;26(2):365-9 - PubMed
5. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed

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