Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli - PubMed (original) (raw)
Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli
S K Levengood et al. J Bacteriol. 1989 Dec.
Abstract
Various mutations in the tolQRAB gene cluster of Escherichia coli render the bacteria tolerant to high concentrations of the E, A, or K colicins as well as tolerant to infection by the single-stranded filamentous bacteriophage. The nucleotide sequence of a 2.8-kilobase fragment containing the tolA and tolB genes was determined. This sequence predicts TolA to be a 421-amino-acid protein of molecular mass 44,190 daltons. Studies using minicells show it to be associated with the inner membrane, presumably via a 21-amino-acid hydrophobic sequence between residues 13 and 35. The remaining 387 residues on the carboxyl side of this region are located in the periplasm. Within this region of TolA is a 230-residue portion that is predicted to form a very long helical segment. This region is rich in alanine, lysine, and glutamic and aspartic acids. The TolB protein is predicted to contain 431 amino acids. Localization studies using minicells show two proteins encoded by this open reading frame. The larger protein of 47.5 kilodaltons appears to be associated with the membrane fractions. The smaller protein is 43 kilodaltons in size and is found with the periplasmic components of the cell.
Similar articles
- Nucleotide sequence of a gene cluster involved in entry of E colicins and single-stranded DNA of infecting filamentous bacteriophages into Escherichia coli.
Sun TP, Webster RE. Sun TP, et al. J Bacteriol. 1987 Jun;169(6):2667-74. doi: 10.1128/jb.169.6.2667-2674.1987. J Bacteriol. 1987. PMID: 3294803 Free PMC article. - Distinct regions of the colicin A translocation domain are involved in the interaction with TolA and TolB proteins upon import into Escherichia coli.
Bouveret E, Rigal A, Lazdunski C, Bénédetti H. Bouveret E, et al. Mol Microbiol. 1998 Jan;27(1):143-57. doi: 10.1046/j.1365-2958.1998.00667.x. Mol Microbiol. 1998. PMID: 9466263 - Isolation and characterization of the Haemophilus influenzae tolQ, tolR, tolA and tolB genes.
Sen K, Sikkema DJ, Murphy TF. Sen K, et al. Gene. 1996 Oct 31;178(1-2):75-81. doi: 10.1016/0378-1119(96)00338-1. Gene. 1996. PMID: 8921895 - Colicin A binds to a novel binding site of TolA in the Escherichia coli periplasm.
Penfold CN, Li C, Zhang Y, Vankemmelbeke M, James R. Penfold CN, et al. Biochem Soc Trans. 2012 Dec 1;40(6):1469-74. doi: 10.1042/BST20120239. Biochem Soc Trans. 2012. PMID: 23176500 Review. - The Tol proteins of Escherichia coli and their involvement in the uptake of biomolecules and outer membrane stability.
Lazzaroni JC, Germon P, Ray MC, Vianney A. Lazzaroni JC, et al. FEMS Microbiol Lett. 1999 Aug 15;177(2):191-7. doi: 10.1111/j.1574-6968.1999.tb13731.x. FEMS Microbiol Lett. 1999. PMID: 10474183 Review.
Cited by
- Antibacterial toxin colicin N and phage protein G3p compete with TolB for a binding site on TolA.
Ridley H, Lakey JH. Ridley H, et al. Microbiology (Reading). 2015 Mar;161(Pt 3):503-15. doi: 10.1099/mic.0.000024. Epub 2014 Dec 23. Microbiology (Reading). 2015. PMID: 25536997 Free PMC article. - The crystal structure of the TolB box of colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins.
Zhang Y, Li C, Vankemmelbeke MN, Bardelang P, Paoli M, Penfold CN, James R. Zhang Y, et al. Mol Microbiol. 2010 Feb;75(3):623-36. doi: 10.1111/j.1365-2958.2009.06808.x. Epub 2009 Jul 21. Mol Microbiol. 2010. PMID: 19627502 Free PMC article. - Interactions of the energy transducer TonB with noncognate energy-harvesting complexes.
Brinkman KK, Larsen RA. Brinkman KK, et al. J Bacteriol. 2008 Jan;190(1):421-7. doi: 10.1128/JB.01093-07. Epub 2007 Oct 26. J Bacteriol. 2008. PMID: 17965155 Free PMC article. - Selection for highly biased amino acid frequency in the TolA cell envelope protein of Proteobacteria.
Rooney AP. Rooney AP. J Mol Evol. 2003 Dec;57(6):731-6. doi: 10.1007/s00239-003-2530-6. J Mol Evol. 2003. PMID: 14745542 - Characterization of divIVA and other genes located in the chromosomal region downstream of the dcw cluster in Streptococcus pneumoniae.
Fadda D, Pischedda C, Caldara F, Whalen MB, Anderluzzi D, Domenici E, Massidda O. Fadda D, et al. J Bacteriol. 2003 Oct;185(20):6209-14. doi: 10.1128/JB.185.20.6209-6214.2003. J Bacteriol. 2003. PMID: 14526035 Free PMC article.
References
- J Bacteriol. 1967 Oct;94(4):1093-111 - PubMed
- Annu Rev Biochem. 1986;55:195-224 - PubMed
- J Bacteriol. 1986 Jan;165(1):107-15 - PubMed
- J Bacteriol. 1973 Aug;115(2):506-13 - PubMed
- Eur J Biochem. 1967 Mar;1(1):3-11 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases