Flagellar mutants of Chlamydomonas: studies of radial spoke-defective strains by dikaryon and revertant analysis - PubMed (original) (raw)
Flagellar mutants of Chlamydomonas: studies of radial spoke-defective strains by dikaryon and revertant analysis
D Luck et al. Proc Natl Acad Sci U S A. 1977 Aug.
Abstract
The motility mutant of Chlamydomonas reinhardtii pf14 lacks radial spoke structures in its flagellar axonemes, and 12 proteins present in wild type are missing from a two-dimensional map (isoelectrofocusing/sodium dodecyl sulfate electrophoresis) of its (35)S-labeled flagellar proteins. Six of these same proteins are missing in pf1, which lacks spoke-heads. To determine whether any of the missing proteins represent the mutant gene product two experimental approaches have been applied. The first makes use of the fact that gametes of either mutant strain when fused with wild-type gametes to form quadriflagellate dikaryons undergo recovery of flagellar function. Recovery at the molecular level was monitored by prelabeling the mutant proteins with (35)S and allowing recovery to occur in the absence of protein synthesis. It is to be expected that the mutant gene product would not be restored as a radioactive protein and that recovery would depend on the assembly of the wild-type counterpart that is not labeled. The second technique makes use of revertants induced by UV irradiation. Dikaryon rescue in the case of pf14 leads to restoration of 11 radioactive components; only protein 3 fails to appear as a radioactive spot. For pf1 only two radioactive proteins are restored; proteins 4, 6, 9, and 10 were not radioactive. Analysis of revertants of pf1 gave evidence (altered map positions) that protein 4 is the mutant gene product. In the case of pf14, analysis of 22 revertants has not provided similar positive evidence that protein 3 is the gene product.
Similar articles
- Molecular cloning and sequence analysis of the Chlamydomonas gene coding for radial spoke protein 3: flagellar mutation pf-14 is an ochre allele.
Williams BD, Velleca MA, Curry AM, Rosenbaum JL. Williams BD, et al. J Cell Biol. 1989 Jul;109(1):235-45. doi: 10.1083/jcb.109.1.235. J Cell Biol. 1989. PMID: 2745550 Free PMC article. - Radial spokes of Chlamydomonas flagella: genetic analysis of assembly and function.
Huang B, Piperno G, Ramanis Z, Luck DJ. Huang B, et al. J Cell Biol. 1981 Jan;88(1):80-8. doi: 10.1083/jcb.88.1.80. J Cell Biol. 1981. PMID: 7204490 Free PMC article. - Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation.
Diener DR, Curry AM, Johnson KA, Williams BD, Lefebvre PA, Kindle KL, Rosenbaum JL. Diener DR, et al. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5739-43. doi: 10.1073/pnas.87.15.5739. Proc Natl Acad Sci U S A. 1990. PMID: 2377611 Free PMC article. - The awesome power of dikaryons for studying flagella and basal bodies in Chlamydomonas reinhardtii.
Dutcher SK. Dutcher SK. Cytoskeleton (Hoboken). 2014 Feb;71(2):79-94. doi: 10.1002/cm.21157. Epub 2013 Dec 12. Cytoskeleton (Hoboken). 2014. PMID: 24272949 Free PMC article. Review. - Organelle size regulation: length matters.
Rosenbaum J. Rosenbaum J. Curr Biol. 2003 Jul 1;13(13):R506-7. doi: 10.1016/s0960-9822(03)00440-8. Curr Biol. 2003. PMID: 12842024 Review.
Cited by
- Cytoplasmic microtubules containing acetylated alpha-tubulin in Chlamydomonas reinhardtii: spatial arrangement and properties.
LeDizet M, Piperno G. LeDizet M, et al. J Cell Biol. 1986 Jul;103(1):13-22. doi: 10.1083/jcb.103.1.13. J Cell Biol. 1986. PMID: 3722261 Free PMC article. - Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii.
Barsel SE, Wexler DE, Lefebvre PA. Barsel SE, et al. Genetics. 1988 Apr;118(4):637-48. doi: 10.1093/genetics/118.4.637. Genetics. 1988. PMID: 3366366 Free PMC article. - Biosynthesis of Linear Protein Nanoarrays Using the Flagellar Axoneme.
Ishikawa H, Tian JL, Yu JE, Marshall WF, Qin H. Ishikawa H, et al. ACS Synth Biol. 2022 Apr 15;11(4):1454-1465. doi: 10.1021/acssynbio.1c00439. Epub 2022 Mar 10. ACS Synth Biol. 2022. PMID: 35271249 Free PMC article. - Ultrastructure of respiratory cilia of WIC-Hyd male rats. An animal model for human immotile cilia syndrome.
Torikata C, Kijimoto C, Koto M. Torikata C, et al. Am J Pathol. 1991 Feb;138(2):341-7. Am J Pathol. 1991. PMID: 1992761 Free PMC article. - Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases.
Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G. Iomini C, et al. J Cell Biol. 2001 Apr 2;153(1):13-24. doi: 10.1083/jcb.153.1.13. J Cell Biol. 2001. PMID: 11285270 Free PMC article.
References
- Genetics. 1943 Nov;28(6):491-511 - PubMed
- Proc Natl Acad Sci U S A. 1963 Nov;50:1002-10 - PubMed
- Annu Rev Microbiol. 1960;14:197-216 - PubMed
- J Gen Microbiol. 1954 Dec;11(3):358-63 - PubMed
- Proc Natl Acad Sci U S A. 1977 Apr;74(4):1600-4 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources