Hormonal effects on intracellular and secreted casein in cultures of mouse mammary epithelial cells on floating collagen membranes - PubMed (original) (raw)

Hormonal effects on intracellular and secreted casein in cultures of mouse mammary epithelial cells on floating collagen membranes

J T Emerman et al. Proc Natl Acad Sci U S A. 1977 Oct.

Abstract

Cultured on floating collagen membranes in the presence of lactogenic hormones, dissociated normal mammary epithelial cells from prelactating mice acquire the ultrastructural and biochemical characteristics of differentiated mammary secretory cells in vivo. The cells on floating collagen membranes in medium containing insulin alone have sparse secretory organelles, and a small amount of casein can be detected in these cells with a sensitive radioimmunoassay. These cells resemble counterpart cells in early-pregnant mice. When the cells are exposed to insulin, cortisol, and prolactin, the secretory apparatus is elaborated and significant increases in intracellular and extracellular casein are observed. In this environment, the intracellular casein content is generally four to eight times greater than in freshly dissociated cells or cells cultured in insulin alone. The amount of casein secreted into the medium by floating-collagen-membrane cultures in the three hormones is from 25 to 200 times greater than that secreted by cultures in insulin alone. Cells cultured on plastic substrates in either hormone combination fail to show any increase in intracellular or extracellular casein. On floating collagen membranes, the cells differentiate in response to hormones as they do in vivo and in organ culture. This cell-culture system provides an opportunity to study direct effects of environmental factors on mammary differentiation at the cellular level.

PubMed Disclaimer

Cited by

The origins of the molecular era of adhesion research.
Horwitz AR. Horwitz AR. Nat Rev Mol Cell Biol. 2012 Dec;13(12):805-11. doi: 10.1038/nrm3473. Epub 2012 Nov 15. Nat Rev Mol Cell Biol. 2012. PMID: 23151664 Free PMC article. Review.
Enhanced melanogenesis of murine melanoma cells cultured on or in collagen gel.
Kono T, Furukawa M, Tanii T, Mizuno N, Taniguchi S, Ishii M, Hamada T. Kono T, et al. Arch Dermatol Res. 1990;282(4):263-6. doi: 10.1007/BF00371647. Arch Dermatol Res. 1990. PMID: 2115320
Phenotypic stability of mouse mammary tumor cells cultured on collagen gels.
Emerman JT, Worth AJ. Emerman JT, et al. In Vitro Cell Dev Biol. 1985 Jan;21(1):49-56. doi: 10.1007/BF02620914. In Vitro Cell Dev Biol. 1985. PMID: 4086425
Basement membrane and apocrine epithelial antigens in differential diagnosis between tubular carcinoma and sclerosing adenosis of the breast.
Ekblom P, Miettinen M, Forsman L, Andersson LC. Ekblom P, et al. J Clin Pathol. 1984 Apr;37(4):357-63. doi: 10.1136/jcp.37.4.357. J Clin Pathol. 1984. PMID: 6323547 Free PMC article.
Extracellular matrix control of mammary gland morphogenesis and tumorigenesis: insights from imaging.
Ghajar CM, Bissell MJ. Ghajar CM, et al. Histochem Cell Biol. 2008 Dec;130(6):1105-18. doi: 10.1007/s00418-008-0537-1. Epub 2008 Nov 14. Histochem Cell Biol. 2008. PMID: 19009245 Free PMC article. Review.

References

1. Gen Comp Endocrinol. 1961 Sep;1:195-210 - PubMed
1. J Natl Cancer Inst. 1963 Feb;30:241-67 - PubMed
1. In Vitro. 1977 May;13(5):316-28 - PubMed
1. J Natl Cancer Inst. 1977 Feb;58(2):419-22 - PubMed
1. Cancer Res. 1970 Jul;30(7):1963-8 - PubMed

Hormonal effects on intracellular and secreted casein in cultures of mouse mammary epithelial cells on floating collagen membranes - PubMed (original) (raw)