The distribution of interspersed repetitive DNA sequences in the human genome - PubMed (original) (raw)
The distribution of interspersed repetitive DNA sequences in the human genome
R K Moyzis et al. Genomics. 1989 Apr.
Abstract
The distribution of interspersed repetitive DNA sequences in the human genome has been investigated, using a combination of biochemical, cytological, computational, and recombinant DNA approaches. "Low-resolution" biochemical experiments indicate that the general distribution of repetitive sequences in human DNA can be adequately described by models that assume a random spacing, with an average distance of 3 kb. A detailed "high-resolution" map of the repetitive sequence organization along 400 kb of cloned human DNA, including 150 kb of DNA fragments isolated for this study, is consistent with this general distribution pattern. However, a higher frequency of spacing distances greater than 9.5 kb was observed in this genomic DNA sample. While the overall repetitive sequence distribution is best described by models that assume a random distribution, an analysis of the distribution of Alu repetitive sequences appearing in the GenBank sequence database indicates that there are local domains with varying Alu placement densities. In situ hybridization to human metaphase chromosomes indicates that local density domains for Alu placement can be observed cytologically. Centric heterochromatin regions, in particular, are at least 50-fold underrepresented in Alu sequences. The observed distribution for repetitive sequences in human DNA is the expected result for sequences that transpose throughout the genome, with local regions of "preference" or "exclusion" for integration.
Similar articles
- Repeated DNA of the human Y chromosome.
Smith KD, Young KE, Talbot CC Jr, Schmeckpeper BJ. Smith KD, et al. Development. 1987;101 Suppl:77-92. doi: 10.1242/dev.101.Supplement.77. Development. 1987. PMID: 2846258 - Interspersed repeated sequences in the African green monkey genome that are homologous to the human Alu family.
Grimaldi G, Queen C, Singer MF. Grimaldi G, et al. Nucleic Acids Res. 1981 Nov 11;9(21):5553-68. doi: 10.1093/nar/9.21.5553. Nucleic Acids Res. 1981. PMID: 6273798 Free PMC article. - Evolution and distribution of (GT)n repetitive sequences in mammalian genomes.
Stallings RL, Ford AF, Nelson D, Torney DC, Hildebrand CE, Moyzis RK. Stallings RL, et al. Genomics. 1991 Jul;10(3):807-15. doi: 10.1016/0888-7543(91)90467-s. Genomics. 1991. PMID: 1909685 - Repetitive sequence fingerprinting in the long range mapping of mammalian genomes.
Hamvas RM, Lehrach HR. Hamvas RM, et al. Electrophoresis. 1995 Sep;16(9):1602-6. doi: 10.1002/elps.11501601264. Electrophoresis. 1995. PMID: 8582341 Review. - Maintenance of function without selection: Alu sequences as "cheap genes".
Zuckerkandl E, Latter G, Jurka J. Zuckerkandl E, et al. J Mol Evol. 1989 Dec;29(6):504-12. doi: 10.1007/BF02602922. J Mol Evol. 1989. PMID: 2515297 Review.
Cited by
- Linkage mapping by simultaneous screening of multiple polymorphic loci using Alu oligonucleotide-directed PCR.
Zietkiewicz E, Labuda M, Sinnett D, Glorieux FH, Labuda D. Zietkiewicz E, et al. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8448-51. doi: 10.1073/pnas.89.18.8448. Proc Natl Acad Sci U S A. 1992. PMID: 1528850 Free PMC article. - Linear decay of retrotransposon antisense bias across genes is contingent upon tissue specificity.
Linker S, Hedges DJ. Linker S, et al. PLoS One. 2013 Nov 14;8(11):e79402. doi: 10.1371/journal.pone.0079402. eCollection 2013. PLoS One. 2013. PMID: 24244495 Free PMC article. - Two Separate Cases: Complex Chromosomal Abnormality Involving Three Chromosomes and Small Supernumerary Marker Chromosome in Patients with Impaired Reproductive Function.
Karamysheva TV, Gayner TA, Muzyka VV, Orishchenko KE, Rubtsov NB. Karamysheva TV, et al. Genes (Basel). 2020 Dec 17;11(12):1511. doi: 10.3390/genes11121511. Genes (Basel). 2020. PMID: 33348590 Free PMC article. - Subchromosomal mapping of a putative transformation suppressor gene on human chromosome 1.
Horikawa I, Yamada H, Kugoh H, Yuasa Y, Suzuki M, Oshimura M. Horikawa I, et al. Jpn J Cancer Res. 1995 May;86(5):444-50. doi: 10.1111/j.1349-7006.1995.tb03077.x. Jpn J Cancer Res. 1995. PMID: 7790318 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources