Comparative transcriptome analysis in induced neural stem cells reveals defined neural cell identities in vitro and after transplantation into the adult rodent brain - PubMed (original) (raw)
doi: 10.1016/j.scr.2016.04.015. Epub 2016 Apr 19.
Marcos J Araúzo-Bravo 2, Christina Zerfass 3, Volker Senner 3, Marc Ehrlich 1, Olympia E Psathaki 4, Dong Wook Han 5, Natalia Tapia 6, Holm Zaehres 4, Hans R Schöler 4, Tanja Kuhlmann 3, Gunnar Hargus 7
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- PMID: 27153350
- DOI: 10.1016/j.scr.2016.04.015
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Comparative transcriptome analysis in induced neural stem cells reveals defined neural cell identities in vitro and after transplantation into the adult rodent brain
Anna-Lena Hallmann et al. Stem Cell Res. 2016 May.
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Abstract
Reprogramming technology enables the production of neural progenitor cells (NPCs) from somatic cells by direct transdifferentiation. However, little is known on how neural programs in these induced neural stem cells (iNSCs) differ from those of alternative stem cell populations in vitro and in vivo. Here, we performed transcriptome analyses on murine iNSCs in comparison to brain-derived neural stem cells (NSCs) and pluripotent stem cell-derived NPCs, which revealed distinct global, neural, metabolic and cell cycle-associated marks in these populations. iNSCs carried a hindbrain/posterior cell identity, which could be shifted towards caudal, partially to rostral but not towards ventral fates in vitro. iNSCs survived after transplantation into the rodent brain and exhibited in vivo-characteristics, neural and metabolic programs similar to transplanted NSCs. However, iNSCs vastly retained caudal identities demonstrating cell-autonomy of regional programs in vivo. These data could have significant implications for a variety of in vitro- and in vivo-applications using iNSCs.
Keywords: Induced neural stem cells; Neural stem cells; Regional programs; Transcriptome; Transplantation.
Copyright © 2016 Roslin Cells Ltd. Published by Elsevier B.V. All rights reserved.
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