The long non-coding RNA CCAT2 is up-regulated in ovarian cancer and associated with poor prognosis - PubMed (original) (raw)

The long non-coding RNA CCAT2 is up-regulated in ovarian cancer and associated with poor prognosis

Shuying Huang et al. Diagn Pathol. 2016.

Abstract

Background: Ovarian cancer is a malignant tumor with a poor prognosis. Accumulating evidence demonstrates that long non-coding RNAs (lncRNAs) are emerging regulators in cancer biology, and can be used as potential biomarkers for cancer diagnosis, prognosis and targeted therapy. The lncRNA CCAT2 (colon cancer associated transcript 2) was recently shown to be involved in several cancers; however, its role in ovarian cancer remains unknown.

Methods: Expression levels of the lncRNA CCAT2 in ovarian cancer tissues, adjacent normal tissues, and cell lines were assessed by quantitative real-time PCR. Then, the associations of CCAT2 expression levels with clinicopathological features and prognosis were evaluated. In addition, CCAT2 functions in tumor progression and invasion were further determined by siRNA-induced CCAT2 silencing in vitro.

Results: Expression levels of the lncRNA CCAT2 in ovarian cancer tissues and cell lines were significantly higher compared with values obtained for adjacent non-tumor tissues and normal ovarian epithelial cells. Interestingly, higher CCAT2 expression levels were associated with a shorter overall survival (P = 0.006) and disease-free survival (P = 0.001) in ovarian cancer patients. In addition, CCAT2 expression was positively correlated with FIGO stage (P = 0.002), tumor grade (P = 0.006) and distant metastasis (P < 0.001). Moreover, CCAT2 knockdown in ovarian cancer cells markedly suppressed cell proliferation, migration, and invasion.

Conclusions: The lncRNA CCAT2 is a novel factor involved in ovarian cancer progression, and constitutes a potential prognostic biomarker and therapeutic target for patients with ovarian cancer.

Keywords: Colon cancer associated transcript 2; LncRNAs; Ovarian cancer; Prognosis.

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Figures

Fig. 1

Fig. 1

CCAT2 gene expression levels in ovarian cancer tissues and cells. Gene expression levels of the lncRNA CCAT2 were assessed by qRT-PCR, with GAPDH as an internal control. a CCAT2 expression levels in ovarian cancer samples were significantly higher than those in adjacent non-tumor tissues. b Higher expression levels of CCAT2 were detected in 4 ovarian cancer cell lines compared with values obtained for the normal human ovarian surface epithelial HOSE 6.3 cell line. Data are mean ± SD from triplicate experiments *P < 0.05

Fig. 2

Fig. 2

Kaplan-Meier survival curves for cervical cancer patients according to CCAT2 gene expression level. OS and DFS of patients with high vs. Low CCAT2 expression levels are shown. a OS of ovarian cancer patients with high CCAT2 expression was significantly poorer compared with rates found in patients with low CCAT2 levels (P < 0.05). b DFS of ovarian cancer patients with high CCAT2 expression was significantly poorer compared with rates in patients with low CCAT2 (P < 0.05)

Fig. 3

Fig. 3

Knockdown of the lncRNA CCAT2 inhibits proliferation, migration and invasion in SKOV3 cells. a qRT-PCR revealed that CCAT2 was efficiently knocked down by treatment with si-CCAT2 in SKOV3 cells. b SKOV3 cells transfected with si-CCAT2 displayed significantly lower proliferation ability compared with those transfected with si-NC. c SKOV3 cells transfected with si-CCAT2 showed markedly lower migration ability compared with those transfected with si-NC. d SKOV3 cells transfected with si-CCAT2 displayed significantly lower invasion ability compared with those transfected with si-NC. Data are mean ± SD from triplicate experiments. *P < 0.05

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References

    1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61:69–90. doi: 10.3322/caac.20107. - DOI - PubMed
    1. Shapira I, Oswald M, Lovecchio J, Khalili H, Menzin A, Whyte J, et al. Circulating biomarkers for detection of ovarian cancer and predicting cancer outcomes. Br J Cancer. 2014;110:976–83. doi: 10.1038/bjc.2013.795. - DOI - PMC - PubMed
    1. Knoll M, Lodish HF, Sun L. Long non-coding RNAs as regulators of the endocrine system. Nat Rev Endocrinol. 2015;11:151–60. doi: 10.1038/nrendo.2014.229. - DOI - PMC - PubMed
    1. Qiu JJ, Wang Y, Ding JX, Jin HY, Yang G, Hua KQ. The long non-coding RNA HOTAIR promotes the proliferation of serous ovarian cancer cells through the regulation of cell cycle arrest and apoptosis. Exp Cell Res. 2015;333:238–48. doi: 10.1016/j.yexcr.2015.03.005. - DOI - PubMed
    1. Lin L, Gu ZT, Chen WH, Cao KJ. Increased expression of the long non-coding RNA ANRIL promotes lung cancer cell metastasis and correlates with poor prognosis. Diagn Pathol. 2015;10:14. doi: 10.1186/s13000-015-0247-7. - DOI - PMC - PubMed

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