Sundarban Honey Confers Protection against Isoproterenol-Induced Myocardial Infarction in Wistar Rats - PubMed (original) (raw)

Sundarban Honey Confers Protection against Isoproterenol-Induced Myocardial Infarction in Wistar Rats

Rizwana Afroz et al. Biomed Res Int. 2016.

Abstract

The present study was designed to investigate the cardioprotective effects of Sundarban honey (SH) in rats with isoproterenol- (ISO-) induced myocardial infarction. Adult male Wistar Albino rats were pretreated with Sundarban honey (5 g/kg) daily for a period of 6 weeks. After the treatment period, ISO (85 mg/kg) was subcutaneously injected into the rats at 24 h intervals for 2 days. ISO-induced myocardial damage was indicated by increased serum cardiac specific troponin I levels and cardiac marker enzyme activities including creatine kinase-MB, lactate dehydrogenase, aspartate transaminase, and alanine transaminase. Significant increases in serum total cholesterol, triglycerides, and low-density lipoprotein-cholesterol levels were also observed, along with a reduction in the serum high-density lipoprotein-cholesterol level. In addition to these diagnostic markers, the levels of lipid peroxide products were significantly increased. The activities of antioxidant enzymes such as superoxide dismutase, glutathione peroxidase, and glutathione reductase were significantly decreased in the hearts after ISO-induced myocardial infarction. However, pretreatment of ischemic rats with Sundarban honey brought the biochemical parameters to near normalcy, indicating the protective effect of Sundarban honey against ISO-induced ischemia in rats. Histopathological findings of the heart tissues further confirmed the biochemical findings, indicating that Sundarban honey confers protection against ISO-induced oxidative stress in the myocardium.

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Figures

Figure 1

Figure 1

Schematic representation of experimental design of the study.

Figure 2

Figure 2

The effects of SH on serum cTn I levels. The bars represent the mean ± SD (n = 8); bars with different letters are significantly different at p < 0.05.

Figure 3

Figure 3

The cardioprotective effects of SH on the activities of cardiac marker enzymes. The bars represent the mean ± SD (n = 8); bars with different letters are significantly different at p < 0.05.

Figure 4

Figure 4

The cardioprotective effects of SH on cardiac LPO levels. The bars represent the mean ± SD (n = 8); bars with different letters are significantly different at p < 0.05.

Figure 5

Figure 5

The effects of SH pretreatment on histopathological changes in the heart tissues of experimental rats. (a) Sham group: normal control rat heart showing normal cardiac muscle fibers; (b) SH + sham group: SH pretreated rat heart showing normal muscle fibers without any pathological changes; (c) SH + ISO group: SH pretreated rat myocardial tissues experiencing ISO challenge showing hyperplastic muscle fibers and decreased degree of inflammatory cells; (d) ISO group: only ISO treated rat heart showing cardiac muscle fibers with muscle separation (red arrows), edematous intramuscular space (green arrows), and inflammatory cell infiltration (yellow arrows). Magnification: 40x.

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