Primary structure analysis of an integral membrane glycoprotein of the nuclear pore - PubMed (original) (raw)
Primary structure analysis of an integral membrane glycoprotein of the nuclear pore
R W Wozniak et al. J Cell Biol. 1989 Jun.
Abstract
The complete primary structure of an integral membrane glycoprotein of the nuclear pore was deduced from the cDNA sequence. The cDNA encodes a polypeptide of 204,205 D containing a 25-residue-long signal sequence, two hydrophobic segments that could function as transmembrane segments, and 13 potential N-linked oligosaccharide addition sites. Endoglycosidase H reduces the molecular mass by approximately 9 kD suggesting that not all of these 13 sites are used. We discuss possible models for the topology of this protein in the pore membrane as well as a possible role in the formation of pores and pore complexes.
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References
- Anal Biochem. 1986 Apr;154(1):353-60 - PubMed
- Science. 1986 Apr 25;232(4749):485-7 - PubMed
- Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 - PubMed
- Methods Enzymol. 1987;152:556-62 - PubMed
- Methods Enzymol. 1987;152:61-87 - PubMed
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