Molecular cloning of a mouse DNA repair gene that complements the defect of group-A xeroderma pigmentosum - PubMed (original) (raw)

Molecular cloning of a mouse DNA repair gene that complements the defect of group-A xeroderma pigmentosum

K Tanaka et al. Proc Natl Acad Sci U S A. 1989 Jul.

Abstract

For isolation of the gene responsible for xeroderma pigmentosum (XP) complementation group A, plasmid pSV2gpt and genomic DNA from a mouse embryo were cotransfected into XP2OSSV cells, a group-A XP cell line. Two primary UV-resistant XP transfectants were isolated from about 1.6 X 10(5) pSV2gpt-transformed XP colonies. pSV2gpt and genomic DNA from the primary transfectants were again cotransfected into XP2OSSV cells and a secondary UV-resistant XP transfectant was obtained by screening about 4.8 X 10(5) pSV2gpt-transformed XP colonies. The secondary transfectant retained fewer mouse repetitive sequences. A mouse gene that complements the defect of XP2OSSV cells was cloned into an EMBL3 vector from the genome of a secondary transfectant. Transfections of the cloned DNA also conferred UV resistance on another group-A XP cell line but not on XP cell lines of group C, D, F, or G. Northern blot analysis of poly(A)+ RNA with a subfragment of cloned mouse DNA repair gene as the probe revealed that an approximately 1.0 kilobase mRNA was transcribed in the donor mouse embryo and secondary transfectant, and approximately 1.0- and approximately 1.3-kilobase mRNAs were transcribed in normal human cells, but none of these mRNAs was detected in three strains of group-A XP cells. These results suggest that the cloned DNA repair gene is specific for group-A XP and may be the mouse homologue of the group-A XP human gene.

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References

1. 1. Gene. 1988 Jun 15;66(1):65-76 - PubMed
2. 1. Cell. 1988 Apr 8;53(1):97-106 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 - PubMed
4. 1. Nat New Biol. 1972 Jul 19;238(81):80-3 - PubMed
5. 1. Proc Natl Acad Sci U S A. 1975 Jan;72(1):219-23 - PubMed

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