Differential Kv1.3, KCa3.1, and Kir2.1 expression in "classically" and "alternatively" activated microglia - PubMed (original) (raw)

Figure 7

Summary of functional K V1.3, K Ca3.1, and K ir2.1 expression levels measured by whole‐cell patch‐clamp 40–48 h after stimulation with the respective stimulus**.** Top: K V1.3 current density at +40 mV for floating microglia (5.0 ± 4.4 pA/pF, n = 35) and microglia treated with IL‐4 (3.0 ± 1.0 pA/pF, n = 15, P = 9.1 x 10−2), LPS (46.0 ± 35.4 pA/pF, n = 33, P = 4.1 x 10−9), LPS + IFN‐γ (43.2 ± 33.6 pA/pF, n = 14, P = 2.6 x 10−8), IFN‐γ (4.2 ± 3.0 pA/pF, n = 41, P = 3.8 x 10−1), MgATP/Na2ATP (3.3 ± 1.5 pA/pF, n = 32, P = 4.1 x 10−2) and PMA/ionomycin (6.8 ± 5.0 pA/pF, n = 12, P = 2.4 x 10−1). Middle: K ir current density at −120 mV for floating microglia (4.2 ± 2.0 pA/pF, n = 30) and microglia treated with IL‐4 (9.4 ± 6.2 pA/pF, n = 20, P = 1.1 x 10−4), LPS (2.8 ± 1.8 pA/pF, n = 21, P = 9.1 x 10−3), LPS + IFN‐γ (2.9 ± 1.5 pA/pF, n = 15, P = 3.0 x 10−2), IFN‐γ (7.6 ± 5.6 pA/pF, n = 50, P = 2.6 x 10 − 3), MgATP/Na2ATP (14.2 ± 7.0 pA/pF, n = 33, P = 2.2 x 10−10) and PMA/ionomycin (9.9 ± 4.8 pA/pF, n = 13, P = 2.4 x 10−6). Bottom) KCa3.1 current density determined from the slope of ramp current between −80 mV to −70 mV: for floating microglia (3.4 ± 3.9 pS/pF, n = 33) and microglia treated with IL‐4 (1.1 ± 1.3 pS/pF, n = 15, P = 2.6 x 10 − 2), LPS (0.7 ± 1.5 pS/pF, n = 20, P = 5.4 x 10 − 3), LPS + IFN‐γ (0.8 ± 1.6 pS/pF, n = 14, P = 2.0 x 10 − 2), IFN‐γ (10.2 ± 11.5 pS/pF, n = 47, P = 1.5 x 10−3), MgATP/Na2ATP (9.4 ± 9.0 pS/pF, n = 32, P = 9.0 x 10−4) and PMA/ionomycin (14.3 ± 17.6 pA/pF, n = 13, P = 1.4 x 10−3). All data are mean ± S.D. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.