Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice - PubMed (original) (raw)

. 2016 Dec;66(12):5452-5459.

doi: 10.1099/ijsem.0.001540. Epub 2016 Oct 4.

Bodo Linz 1 2, Karen B Register 3, Jeffrey D Newman 4, Dawn L Taylor 1 2, Kenneth R Boschert 5, Soazig Le Guyon 6, Emily F Wilson 1, Lauren M Brinkac 7, Ravi Sanka 7, Suellen C Greco 5, Paula M Klender 5, Liliana Losada 7, Eric T Harvill 1 2 6

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Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice

Yury V Ivanov et al. Int J Syst Evol Microbiol. 2016 Dec.

Abstract

Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii-like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii, one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA-DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii - from poultry, humans and rabbit - and Bordetella pseudohinzii sp. nov. type strain 8-296-03T (=NRRL B-59942T=NCTC 13808T) that infect mice.

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Figures

Fig. 1.

Fig. 1.

Phylogenetic structure (neighbour-joining trees) according to (a) a genome-wide sequence alignment and (b) 16S rRNA gene sequences of

Bordetella species. The (GenBank/EMBL/DDBJ/PIR) accession numbers for the Whole Genome Shotgun sequences of B. hinzii strain LMG 13501T, Bordetella pseudohinzii strain 8-296-03T, Bordetella pseudohinzii strain CWR-1 and Bordetella pseudohinzii strain 228/11 are LRUJ00000000, JHEP00000000, LRSQ00000000 and LRSP00000000, respectively. Accession numbers for genomes of all other species and strains are listed in Table S1.

Fig. 2.

Fig. 2.

Colonization of murine lungs with either

B. pseudohinzii or B. hinzii 7 days after intranasal inoculation. The dashed line is the limit of detection. The hosts of origin for the B. hinzii isolates tested are indicated by H (human), R (rabbit) and P (poultry). B. pseudohinzii isolates originated from mouse (M).

Fig. 3.

Fig. 3.

Comparative gene content analysis of

B. pseudohinzii and B. hinzii genomes. Venn diagram compares core genes of nine B. hinzii strains (LMG 13501T; F582; H568; 1277; L60; OH87 BAL007II; 5132; 4161; CA90 BAL1384) with core genes of three B. pseudohinzii strains (8-296-03T; 228/11; CWR-1). Numbers correspond to core gene families in common (intersection) or unique-to-species core genes (symmetric difference).

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