A dense core vesicle protein is restricted to the cortex of granules in the exocrine atrial gland of Aplysia california - PubMed (original) (raw)
. 1989 Oct 5;264(28):16933-40.
Affiliations
- PMID: 2777814
Free article
A dense core vesicle protein is restricted to the cortex of granules in the exocrine atrial gland of Aplysia california
W S Sossin et al. J Biol Chem. 1989.
Free article
Abstract
We have generated a monoclonal antibody (mAb) 5E10 which recognizes an antigen localized to dense core vesicles (DCVs) in the atrial gland of Aplysia californica. mAb5E10 immunoprecipitates an abundant 57-kDa glycoprotein (atrial gland granule-specific antigen, AGSA) which is a soluble component of atrial gland DCVs. Electron microscopy reveals that AGSA immunoreactivity is restricted to the region between the dense core, which contains neuropeptide immunoreactivity, and the membrane of atrial gland DCVs. AGSA was purified by immunoaffinity chromatography, and the amino acid sequences of both N-terminal and internal cyanogen bromide fragments were determined. This information was used to isolate a 2.8-kilobase cDNA which encodes a 47-kDa protein. The predicted amino acid sequence contains the micro-sequenced peptides, an N-terminal hydrophobic signal sequence, and four N-linked glycosylation sites, but does not contain any significant homologies to database sequences. Northern blots and light level immunocytochemistry demonstrate that the AGSA gene is specifically expressed in the atrial gland. The identification of a protein localized to the cortex of DCVs suggests that this region has a specialized role in the function of these vesicles.
Similar articles
- Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica.
van Heumen WR, Nagle GT, Kurosky A. van Heumen WR, et al. Cell Tissue Res. 1995 Jan;279(1):13-24. doi: 10.1007/BF00300687. Cell Tissue Res. 1995. PMID: 7895254 - A bag cell neuron-specific antigen localizes to a subset of dense core vesicles in Aplysia californica.
Sossin WS, Scheller RH. Sossin WS, et al. Brain Res. 1989 Aug 14;494(2):205-14. doi: 10.1016/0006-8993(89)90588-x. Brain Res. 1989. PMID: 2776014 - Molecular cloning, cDNA sequence, and localization of a prohormone convertase (PC2) from the Aplysia atrial gland.
Nagle GT, Garcia AT, Knock SL, Gorham EL, Van Heumen WR, Kurosky A. Nagle GT, et al. DNA Cell Biol. 1995 Feb;14(2):145-54. doi: 10.1089/dna.1995.14.145. DNA Cell Biol. 1995. PMID: 7865132 - Molecular aspects of egg-laying behavior in Aplysia californica.
DesGroseillers L. DesGroseillers L. Behav Genet. 1990 Mar;20(2):251-64. doi: 10.1007/BF01067793. Behav Genet. 1990. PMID: 2191647 Review. - Neuropeptide genes mediating complex behavior in Aplysia.
Axel R. Axel R. Harvey Lect. 1983-1984;79:1-30. Harvey Lect. 1983. PMID: 6400202 Review. No abstract available.
Cited by
- Adenosine deaminase-related growth factors stimulate cell proliferation in Drosophila by depleting extracellular adenosine.
Zurovec M, Dolezal T, Gazi M, Pavlova E, Bryant PJ. Zurovec M, et al. Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4403-8. doi: 10.1073/pnas.062059699. Epub 2002 Mar 19. Proc Natl Acad Sci U S A. 2002. PMID: 11904370 Free PMC article. - Analysis of the cat eye syndrome critical region in humans and the region of conserved synteny in mice: a search for candidate genes at or near the human chromosome 22 pericentromere.
Footz TK, Brinkman-Mills P, Banting GS, Maier SA, Riazi MA, Bridgland L, Hu S, Birren B, Minoshima S, Shimizu N, Pan H, Nguyen T, Fang F, Fu Y, Ray L, Wu H, Shaull S, Phan S, Yao Z, Chen F, Huan A, Hu P, Wang Q, Loh P, Qi S, Roe BA, McDermid HE. Footz TK, et al. Genome Res. 2001 Jun;11(6):1053-70. doi: 10.1101/gr.154901. Genome Res. 2001. PMID: 11381032 Free PMC article. - Separation and characterization of amines from individual atrial gland vesicles of Aplysia californica.
Lillard SJ, Chiu DT, Scheller RH, Zare RN, Rodríguez-Cruz SE, Williams ER, Orwar O, Sandberg M, Lundqvist JA. Lillard SJ, et al. Anal Chem. 1998 Sep 1;70(17):3517-24. doi: 10.1021/ac980462j. Anal Chem. 1998. PMID: 9737203 Free PMC article. - Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica.
van Heumen WR, Nagle GT, Kurosky A. van Heumen WR, et al. Cell Tissue Res. 1995 Jan;279(1):13-24. doi: 10.1007/BF00300687. Cell Tissue Res. 1995. PMID: 7895254