Toxoplasma antigens recognized by immunoglobulin G subclasses during acute and chronic infection - PubMed (original) (raw)

Comparative Study

Toxoplasma antigens recognized by immunoglobulin G subclasses during acute and chronic infection

J Huskinson et al. J Clin Microbiol. 1989 Sep.

Abstract

The immunoglobulin G (IgG) subclass response to Toxoplasma gondii antigens during the acute and chronic stages of T. gondii infection were studied by using immunoblots with reduced antigen (RA) and nonreduced antigen (NRA) preparations. Serum samples were from individuals with acute or chronic T. gondii infection, and sequential samples were from women who seroconverted during gestation and were treated with spiramycin. IgG1 antibodies were predominant in sera from each of the groups and recognized a large number of RA and NRA. In the latter group of patients, IgG1 and IgG3 were the first antibodies to appear in response to the infection. In all groups, an antigen with a molecular weight (MW) of 30,000 was the most intensely stained and frequently recognized by IgG1 antibodies in NRA preparations. In RA preparations, antigens of MW 35,000 and 30,000 were the most intensely stained and frequently recognized by IgG1 antibodies. An antigen with an MW of 22,000 was intensely stained in the IgG1 immunoblots of the NRA preparation and to a lesser extent in the RA preparation. In contrast to immunoblots with IgG1 antibodies, very few antigens in the RA and NRA preparations were detected by IgG2 and IgG3 antibodies; IgG4 antibodies rarely detected any antigens. Of interest was that IgG2 antibodies detected antigens distributed over the entire MW range, whereas those detected by IgG3 antibodies were located mostly below the 35,000 MW marker. Enzyme-linked immunosorbent assay results paralleled those of the immunoblots in that IgG1 antibodies were predominant.

PubMed Disclaimer

Cited by

Evaluation of Specific Cellular and Humoral Immune Response to Toxoplasma gondii in Patients with Autoimmune Rheumatic Diseases Immunomodulated Due to the Use of TNF Blockers.
Andrade CMR, de Lima Marques AC, Timóteo RP, de Morais Oliveira-Scussel AC, De Vito FB, da Silva MV, Mineo JR, Teodoro RB, Rodrigues DBR, Júnior VR. Andrade CMR, et al. Biomedicines. 2023 Mar 17;11(3):930. doi: 10.3390/biomedicines11030930. Biomedicines. 2023. PMID: 36979909 Free PMC article.
Association of gestational diabetes mellitus and negative modulation of the specific humoral and cellular immune response against Toxoplasma gondii.
Oliveira-Scussel ACM, Ferreira PTM, Resende RS, Ratkevicius-Andrade CM, Gomes AO, Paschoini MC, De Vito FB, Farnesi-de-Assunção TS, da Silva MV, Mineo JR, Rodrigues DBR, Rodrigues V. Oliveira-Scussel ACM, et al. Front Immunol. 2022 Sep 20;13:925762. doi: 10.3389/fimmu.2022.925762. eCollection 2022. Front Immunol. 2022. PMID: 36203592 Free PMC article.
Comparative Detection of Immunoglobulin Isotypes and Subclasses against Toxoplasma gondii Soluble Antigen in Serum and Colostrum Samples from Puerperal Women.
Borges HDS, Oliveira-Scussel ACM, Oliveira ÂMM, Abdallah VOS, Pajuaba ACAM, Mineo JR. Borges HDS, et al. Int J Environ Res Public Health. 2022 Jun 29;19(13):7953. doi: 10.3390/ijerph19137953. Int J Environ Res Public Health. 2022. PMID: 35805611 Free PMC article.
Species-specific differences in Toxoplasma gondii, Neospora caninum and Besnoitia besnoiti seroprevalence in Namibian wildlife.
Seltmann A, Schares G, Aschenborn OHK, Heinrich SK, Thalwitzer S, Wachter B, Czirják GÁ. Seltmann A, et al. Parasit Vectors. 2020 Jan 8;13(1):7. doi: 10.1186/s13071-019-3871-3. Parasit Vectors. 2020. PMID: 31915056 Free PMC article.

References

1. J Immunol. 1988 Aug 1;141(3):978-83 - PubMed
1. J Clin Microbiol. 1983 Jul;18(1):63-70 - PubMed
1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
1. J Infect Dis. 1980 Feb;141(2):144-50 - PubMed
1. N Engl J Med. 1973 Oct 25;289(17):878-81 - PubMed

Toxoplasma antigens recognized by immunoglobulin G subclasses during acute and chronic infection - PubMed (original) (raw)