Microbial-type terpene synthase genes occur widely in nonseed land plants, but not in seed plants - PubMed (original) (raw)

Fig. 4.

Representative MTPSL genes from four groups (I–IV) encode active TPSs. The aspartate-rich motif associated with each group is indicated. MTPSL genes were heterologously expressed in E. coli and crude protein extracts were incubated with the potential substrate farnesyl diphosphate [(E,E)-FPP]. Enzyme products were collected using solid-phase microextraction and analyzed with gas chromatography/mass spectrometry. 1, bicycloelemene; 2, bicyclogermacrene; 3, α-isocomene; 4, β-elemene*; 5, (E)-β-caryophyllene*; 6, (E)-β-farnesene*; 7, nerolidol*; 8, dactylol; 9, γ-curcumene; 10, α-zingiberene; 11, β-bisabolene*; 12, β-curcumene; 13, sesquiphellandrene*; 14, (E)-α-bisabolene; 15, (Z,E)-α-farnesene; 16, (E,E)-α-farnesene; 17, protoillud-6-ene*; 18, (Z)-γ-bisabolene; 19, (E)-γ-bisabolene; 20, β-bisabolol; 21, α-bisabolol; and 22, β-acoradiene. Compounds marked with an asterisk (*) were identified using authentic standards. The origin of each MTPSL gene is listed in SI Appendix, Table S8. Ap, A. punctatus; GOWD, S. lescurii; GSXD, M. eatonii; Hon, hornwort; IRBN, S. nemorea; Liv, liverwort; Mon, monilophyte; Mos, moss; QKQO, Pseudotaxiphyllum elegans; UJTT, Pityrogramma trifoliate; VBMM, Anomodon rostratus; and YJJY, Woodsia scopulina.