Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow - PubMed (original) (raw)

Affiliations

PMID: 2783573

Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow

K Itoh et al. Exp Hematol. 1989 Feb.

Abstract

Stromal cell lines, designated MS-1, -2, -3, -4, -5, -6, and -7 were established by irradiating the adherent cells in long-term bone marrow cultures with 900-rad x-rays. Two of the cell lines, MS-1 and MS-5, have the capacity to support the growth of hemopoietic stem cells (spleen colony-forming cells and granulocyte-macrophage colony-forming cells) for greater than 2 months in vitro. These two cell lines were alkaline phosphatase-, peroxidase-, and factor VIII-negative and positive for periodic acid-Schiff and nonspecific esterase. Extracellular matrix proteins such as fibronectin, laminin, and collagen type I were produced by these two cell lines. Neither MS-1 cell- nor MS-5 cell-conditioned medium supported the growth of hemopoietic stem cells, and hemopoietic stem cells were found preferentially to be under and on MS-1 and MS-5 layers rather than in suspension. Close contact with the MS-1 cell layer or the MS-5 cell layer appears to be essential in maintaining hemopoiesis in vitro. Conditioned media from MS-1 cells and MS-5 cells stimulated granulocyte colony formation from murine bone marrow cells in semisolid culture.

PubMed Disclaimer

Cited by

Efficient generation of human NOTCH ligand-expressing haemogenic endothelial cells as infrastructure for in vitro haematopoiesis and lymphopoiesis.
Sun S, Motazedian A, Li JY, Wijanarko K, Zhu JJ, Tharmarajah K, Strumila KA, Shkaruta A, Nigos LR, Schiesser JV, Yu Y, Neeson PJ, Ng ES, Elefanty AG, Stanley EG. Sun S, et al. Nat Commun. 2024 Sep 4;15(1):7698. doi: 10.1038/s41467-024-51974-7. Nat Commun. 2024. PMID: 39227582 Free PMC article.
In vivo repopulation ability of genetically corrected bone marrow cells from Fanconi anemia patients.
Cohen-Haguenauer O, Péault B, Bauche C, Daniel MT, Casal I, Levy V, Dausset J, Boiron M, Auclair C, Gluckman E, Marty M. Cohen-Haguenauer O, et al. Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2340-5. doi: 10.1073/pnas.0510613103. Epub 2006 Feb 6. Proc Natl Acad Sci U S A. 2006. PMID: 16461901 Free PMC article.
Expression of glypican-4 in haematopoietic-progenitor and bone-marrow-stromal cells.
Siebertz B, Stöcker G, Drzeniek Z, Handt S, Just U, Haubeck HD. Siebertz B, et al. Biochem J. 1999 Dec 15;344 Pt 3(Pt 3):937-43. Biochem J. 1999. PMID: 10585884 Free PMC article.
Lymphoid cell development from fetal hematopoietic progenitors and human pluripotent stem cells.
Sun S, Wijanarko K, Liani O, Strumila K, Ng ES, Elefanty AG, Stanley EG. Sun S, et al. Immunol Rev. 2023 May;315(1):154-170. doi: 10.1111/imr.13197. Epub 2023 Mar 20. Immunol Rev. 2023. PMID: 36939073 Free PMC article. Review.
Intrathymic dendritic cell-biased precursors promote human T cell lineage specification through IRF8-driven transmembrane TNF.
Liang KL, Roels J, Lavaert M, Putteman T, Boehme L, Tilleman L, Velghe I, Pegoretti V, Van de Walle I, Sontag S, Vandewalle J, Vandekerckhove B, Leclercq G, Van Vlierberghe P, Libert C, Van Nieuwerburgh F, Fischer R, Kontermann RE, Pfizenmaier K, Doody G, Zenke M, Taghon T. Liang KL, et al. Nat Immunol. 2023 Mar;24(3):474-486. doi: 10.1038/s41590-022-01417-6. Epub 2023 Jan 26. Nat Immunol. 2023. PMID: 36703005

MeSH terms

Substances

LinkOut - more resources

Other Literature Sources
- The Lens - Patent Citations Database
Medical
- MedlinePlus Health Information
Research Materials
- Cellosaurus - a cell line knowledge resource

Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow - PubMed (original) (raw)