Identification of proteins specific for human herpesvirus 6-infected human T cells - PubMed (original) (raw)

Identification of proteins specific for human herpesvirus 6-infected human T cells

N Balachandran et al. J Virol. 1989 Jun.

Abstract

Proteins specific for human herpesvirus 6 (HHV-6)-infected human T cells (HSB-2) were examined by using polyclonal rabbit antibodies and monoclonal antibodies against HHV-6-infected cells and human sera. More than 20 proteins and six glycoproteins specific for HHV-6-infected cells were identified from [35S]methionine- and [3H]glucosamine-labeled total-cell extracts. Polyclonal rabbit antibodies immunoprecipitated 33 [35S]methionine-labeled HHV-6-specific polypeptides with approximate molecular weights ranging from 180,000 to 31,000. In immunoprecipitation and Western immunoblot reactions, a patient's serum also recognized more than 30 HHV-6-specific proteins and seven glycoproteins. In contrast, sera from individuals with high-titered antibodies against other human herpesviruses reacted with fewer HHV-6-infected cell proteins, and only a 135,000-Mr polypeptide was prominent. Monoclonal antibodies to HHV-6-infected cells reacted with single and multiple polypeptides specific for virus-infected cells and immunoprecipitated three distinct sets of glycoproteins, which were designated gp105k and gp82k, gp116k, gp64k, and gp54k, and gp102k.

PubMed Disclaimer

Similar articles

• Human herpesvirus 7: antigenic properties and prevalence in children and adults.
  Wyatt LS, Rodriguez WJ, Balachandran N, Frenkel N. Wyatt LS, et al. J Virol. 1991 Nov;65(11):6260-5. doi: 10.1128/JVI.65.11.6260-6265.1991. J Virol. 1991. PMID: 1656093 Free PMC article.
• Electrophoretic analysis of human herpesvirus 6 polypeptides immunoprecipitated from infected cells with human sera.
  Balachandran N, Tirawatnapong S, Pfeiffer B, Ablashi DV, Salahuddin SZ. Balachandran N, et al. J Infect Dis. 1991 Jan;163(1):29-34. doi: 10.1093/infdis/163.1.29. J Infect Dis. 1991. PMID: 1845808
• Polyvalent and monoclonal antibodies identify major immunogenic proteins specific for human herpesvirus 7-infected cells and have weak cross-reactivity with human herpesvirus 6.
  Foà-Tomasi L, Avitabile E, Ke L, Campadelli-Fiume G. Foà-Tomasi L, et al. J Gen Virol. 1994 Oct;75 ( Pt 10):2719-27. doi: 10.1099/0022-1317-75-10-2719. J Gen Virol. 1994. PMID: 7931158
• Immunogenic glycoproteins of infectious laryngotracheitis herpesvirus.
  York JJ, Sonza S, Fahey KJ. York JJ, et al. Virology. 1987 Dec;161(2):340-7. doi: 10.1016/0042-6822(87)90126-7. Virology. 1987. PMID: 2825407
• Neutralizing monoclonal antibodies directed to infectious bovine rhinotracheitis virus.
  Chang LW, Zee YC, Pritchett RF, Ardans AA. Chang LW, et al. Arch Virol. 1986;88(3-4):203-15. doi: 10.1007/BF01310875. Arch Virol. 1986. PMID: 3010905

Cited by

• Human herpesvirus 7: antigenic properties and prevalence in children and adults.
  Wyatt LS, Rodriguez WJ, Balachandran N, Frenkel N. Wyatt LS, et al. J Virol. 1991 Nov;65(11):6260-5. doi: 10.1128/JVI.65.11.6260-6265.1991. J Virol. 1991. PMID: 1656093 Free PMC article.
• Prevalence of human herpesvirus 6 variant A and B infections in bone marrow transplant recipients as determined by polymerase chain reaction and sequence-specific oligonucleotide probe hybridization.
  Drobyski WR, Eberle M, Majewski D, Baxter-Lowe LA. Drobyski WR, et al. J Clin Microbiol. 1993 Jun;31(6):1515-20. doi: 10.1128/jcm.31.6.1515-1520.1993. J Clin Microbiol. 1993. PMID: 8391023 Free PMC article.
• Definition of a divergent epitope that allows differential detection of early protein p41 from human herpesvirus 6 variants A and B.
  Xu Y, Linde A, Dahl H, Winberg G. Xu Y, et al. J Clin Microbiol. 2001 Apr;39(4):1449-55. doi: 10.1128/JCM.39.4.1449-1455.2001. J Clin Microbiol. 2001. PMID: 11283070 Free PMC article.
• Detection of human herpesvirus-6 in mesial temporal lobe epilepsy surgical brain resections.
  Donati D, Akhyani N, Fogdell-Hahn A, Cermelli C, Cassiani-Ingoni R, Vortmeyer A, Heiss JD, Cogen P, Gaillard WD, Sato S, Theodore WH, Jacobson S. Donati D, et al. Neurology. 2003 Nov 25;61(10):1405-11. doi: 10.1212/01.wnl.0000094357.10782.f9. Neurology. 2003. PMID: 14638964 Free PMC article.
• Human herpesviruses in chronic fatigue syndrome.
  Wallace HL 2nd, Natelson B, Gause W, Hay J. Wallace HL 2nd, et al. Clin Diagn Lab Immunol. 1999 Mar;6(2):216-23. doi: 10.1128/CDLI.6.2.216-223.1999. Clin Diagn Lab Immunol. 1999. PMID: 10066657 Free PMC article.

References

1. 1. J Virol. 1982 Oct;44(1):344-55 - PubMed
2. 1. J Virol Methods. 1988 Sep;21(1-4):29-48 - PubMed
3. 1. Science. 1986 Oct 31;234(4776):596-601 - PubMed
4. 1. Science. 1986 Oct 31;234(4776):601-3 - PubMed
5. 1. J Virol. 1986 Nov;60(2):369-75 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central