Restoration of T-cell function in nude mice by grafting the epitheliomesenchymal thymic rudiment from 10-day-old euthymic embryos - PubMed (original) (raw)

Restoration of T-cell function in nude mice by grafting the epitheliomesenchymal thymic rudiment from 10-day-old euthymic embryos

I Khazaal et al. Cell Differ Dev. 1989 May.

Abstract

The capacity of the uncolonized thymic epithelium to restore immune function in nude mice was demonstrated by grafting the 3rd branchial arch area taken from euthymic 10-day BALB/c embryos into syngeneic newborn nude mice. Twenty-six percent of the operated animals became immunocompetent. T-cell function was tested with skin grafts and the presence of high levels of Thy-1 positive cells plus a variety of in vitro culture assays: Con A stimulation of T lymphocytes, cytotoxicity and alloreactivity in MLR of the recipient toward allogeneic spleen cells. All these tests showed a pattern of response similar to normal euthymic BALB/c mice.

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