The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties - PubMed (original) (raw)
The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties
L Tora et al. EMBO J. 1989 Jul.
Abstract
We demonstrate here that the human oestrogen receptor (hER) cDNA clone pOR8 obtained from MCF-7 cells contains an artefactual point mutation which results in the substitution of a valine for a glycine at amino acid position 400 (Gly-400----Val-400). This mutation in the hormone binding domain of the cloned hER destabilizes its structure and decreases its apparent affinity for oestradiol at 25 degrees C, but not at 4 degrees C, when compared with the wild-type hER with a Gly-400.
Similar articles
- Cloning and characterization of a 77-kDa oestrogen receptor isolated from a human breast cancer cell line.
Pink JJ, Fritsch M, Bilimoria MM, Assikis VJ, Jordan VC. Pink JJ, et al. Br J Cancer. 1997;75(1):17-27. doi: 10.1038/bjc.1997.4. Br J Cancer. 1997. PMID: 9000593 Free PMC article. - Characterization of a point mutation in the hormone binding domain of the estrogen receptor from an breast tumor.
England GM, Bilimoria MM, Chen Z, Assikis VJ, Muenzner HD, Jordan VC. England GM, et al. Int J Oncol. 1998 May;12(5):981-6. doi: 10.3892/ijo.12.5.981. Int J Oncol. 1998. PMID: 9538117 - Oestrogen receptor gene structure and function in breast cancer.
Watts CK, Handel ML, King RJ, Sutherland RL. Watts CK, et al. J Steroid Biochem Mol Biol. 1992 Mar;41(3-8):529-36. doi: 10.1016/0960-0760(92)90378-v. J Steroid Biochem Mol Biol. 1992. PMID: 1562523 - Hormone receptors in breast carcinomas.
Mandal AK. Mandal AK. Indian J Pathol Microbiol. 1995 Jan;38(1):109-18. Indian J Pathol Microbiol. 1995. PMID: 8919478 Review. No abstract available. - Transcriptional activation of the estrogen receptor.
Wei LL. Wei LL. Clin Chem. 1993 Feb;39(2):341-5. Clin Chem. 1993. PMID: 8432025 Review.
Cited by
- CRISPR-Cas9 screen reveals a role of purine synthesis for estrogen receptor α activity and tamoxifen resistance of breast cancer cells.
Hany D, Vafeiadou V, Picard D. Hany D, et al. Sci Adv. 2023 May 12;9(19):eadd3685. doi: 10.1126/sciadv.add3685. Epub 2023 May 12. Sci Adv. 2023. PMID: 37172090 Free PMC article. - In Vitro Generation of Murine Dendritic Cells from Hoxb8-Immortalized Hematopoietic Progenitors.
Häcker H. Häcker H. Methods Mol Biol. 2023;2618:93-107. doi: 10.1007/978-1-0716-2938-3_7. Methods Mol Biol. 2023. PMID: 36905511 - Network-informed discovery of multidrug combinations for ERα+/HER2-/PI3Kα-mutant breast cancer.
Hany D, Zoetemelk M, Bhattacharya K, Nowak-Sliwinska P, Picard D. Hany D, et al. Cell Mol Life Sci. 2023 Mar 3;80(3):80. doi: 10.1007/s00018-023-04730-x. Cell Mol Life Sci. 2023. PMID: 36869202 Free PMC article. - A phospho-tyrosine-based signaling module using SPOP, CSK, and LYN controls TLR-induced IRF activity.
Tawaratsumida K, Redecke V, Wu R, Kuriakose J, Bouchard JJ, Mittag T, Lohman BK, Mishra A, High AA, Häcker H. Tawaratsumida K, et al. Sci Adv. 2022 Jul 8;8(27):eabq0084. doi: 10.1126/sciadv.abq0084. Epub 2022 Jul 8. Sci Adv. 2022. PMID: 35857476 Free PMC article. - Cooperative interaction between ERα and the EMT-inducer ZEB1 reprograms breast cancer cells for bone metastasis.
Mohammadi Ghahhari N, Sznurkowska MK, Hulo N, Bernasconi L, Aceto N, Picard D. Mohammadi Ghahhari N, et al. Nat Commun. 2022 Apr 19;13(1):2104. doi: 10.1038/s41467-022-29723-5. Nat Commun. 2022. PMID: 35440541 Free PMC article.
References
- Adv Protein Chem. 1981;34:167-339 - PubMed
- EMBO J. 1988 Oct;7(10):3037-44 - PubMed
- J Bacteriol. 1983 Jan;153(1):163-8 - PubMed
- J Steroid Biochem. 1984 Jan;20(1):51-6 - PubMed
- Nucleic Acids Res. 1985 May 10;13(9):3305-16 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases