Expression and stability of the Mx protein in different tissues of mice, in response to interferon inducers or to influenza virus infection - PubMed (original) (raw)

Expression and stability of the Mx protein in different tissues of mice, in response to interferon inducers or to influenza virus infection

M A Horisberger et al. J Interferon Res. 1989 Oct.

Abstract

We have defined some characteristics of the mouse Mx protein as a marker of biological response to interferon (IFN) and to virus infection in A2G mice. The Mx protein has been detected and quantitated by Western immunoblot analysis. Upon induction by poly(I):poly(C) or with Newcastle disease virus, the Mx protein is expressed and accumulated in a variety of organs, such as liver, lungs, spleen, kidneys, heart, and brain. In some organs the expression of the Mx protein is detected readily, as soon as 4 h after treatment. The highest protein level is reached at 24 h, and it remains stable for several days declining slowly to return to preinduced levels 2-3 weeks after treatment. Infection with an hepatotropic or a pneumotropic strain of influenza virus resulted in a systemic induction of Mx protein, the highest levels being found in the target organ for virus replication. Our results indicate that the Mx protein is a sensitive, quantitative, and stable marker to follow IFN activity or virus infection in an animal model.

PubMed Disclaimer

Similar articles

• Resistance to influenza virus infection of Mx transgenic mice expressing Mx protein under the control of two constitutive promoters.
  Kolb E, Laine E, Strehler D, Staeheli P. Kolb E, et al. J Virol. 1992 Mar;66(3):1709-16. doi: 10.1128/JVI.66.3.1709-1716.1992. J Virol. 1992. PMID: 1371172 Free PMC article.
• Regulation of the interferon-inducible IFI-78K gene, the human equivalent of the murine Mx gene, by interferons, double-stranded RNA, certain cytokines, and viruses.
  Goetschy JF, Zeller H, Content J, Horisberger MA. Goetschy JF, et al. J Virol. 1989 Jun;63(6):2616-22. doi: 10.1128/JVI.63.6.2616-2622.1989. J Virol. 1989. PMID: 2542574 Free PMC article.
• Studies in the in vivo expression of the influenza resistance gene Mx by in-situ hybridisation.
  Chang KC, Goldspink G, Lida J. Chang KC, et al. Arch Virol. 1990;110(3-4):151-64. doi: 10.1007/BF01311285. Arch Virol. 1990. PMID: 2317151
• Mx proteins: GTPases involved in the interferon-induced antiviral state.
  Pavlovic J, Schröder A, Blank A, Pitossi F, Staeheli P. Pavlovic J, et al. Ciba Found Symp. 1993;176:233-43; discussion 243-7. doi: 10.1002/9780470514450.ch15. Ciba Found Symp. 1993. PMID: 7507812 Review.
• Interferons, Mx genes, and resistance to influenza virus.
  Horisberger MA. Horisberger MA. Am J Respir Crit Care Med. 1995 Oct;152(4 Pt 2):S67-71. doi: 10.1164/ajrccm/152.4_Pt_2.S67. Am J Respir Crit Care Med. 1995. PMID: 7551417 Review.

Cited by

• Cloning and sequence analyses of cDNAs for interferon- and virus-induced human Mx proteins reveal that they contain putative guanine nucleotide-binding sites: functional study of the corresponding gene promoter.
  Horisberger MA, McMaster GK, Zeller H, Wathelet MG, Dellis J, Content J. Horisberger MA, et al. J Virol. 1990 Mar;64(3):1171-81. doi: 10.1128/JVI.64.3.1171-1181.1990. J Virol. 1990. PMID: 2154602 Free PMC article.
• Dynamin-like MxA GTPase: structural insights into oligomerization and implications for antiviral activity.
  Haller O, Gao S, von der Malsburg A, Daumke O, Kochs G. Haller O, et al. J Biol Chem. 2010 Sep 10;285(37):28419-24. doi: 10.1074/jbc.R110.145839. Epub 2010 Jun 10. J Biol Chem. 2010. PMID: 20538602 Free PMC article. Review.
• Molecular cloning of the pufferfish (Takifugu rubripes) Mx gene and functional characterization of its promoter.
  Yap WH, Tay A, Brenner S, Venkatesh B. Yap WH, et al. Immunogenetics. 2003 Jan;54(10):705-13. doi: 10.1007/s00251-002-0525-x. Epub 2003 Jan 9. Immunogenetics. 2003. PMID: 12557057
• Innate Immune Responses to Avian Influenza Viruses in Ducks and Chickens.
  Evseev D, Magor KE. Evseev D, et al. Vet Sci. 2019 Jan 10;6(1):5. doi: 10.3390/vetsci6010005. Vet Sci. 2019. PMID: 30634569 Free PMC article. Review.
• IFN and cytokine responses in ducks to genetically similar H5N1 influenza A viruses of varying pathogenicity.
  Saito LB, Diaz-Satizabal L, Evseev D, Fleming-Canepa X, Mao S, Webster RG, Magor KE. Saito LB, et al. J Gen Virol. 2018 Apr;99(4):464-474. doi: 10.1099/jgv.0.001015. Epub 2018 Feb 12. J Gen Virol. 2018. PMID: 29458524 Free PMC article.

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database