Wall teichoic acid is an essential component of Staphylococcus aureus for the induction of human dendritic cell maturation - PubMed (original) (raw)
Wall teichoic acid is an essential component of Staphylococcus aureus for the induction of human dendritic cell maturation
Sung Jun Hong et al. Mol Immunol. 2017 Jan.
Abstract
Staphylococcus aureus is a Gram-positive pathogen that can cause chronic skin inflammation, pneumonia, and septic shock. The immunomodulatory functions of wall teichoic acid (WTA), a glycopolymer abundantly expressed on the Gram-positive bacterial cell wall, are poorly understood. Here, we investigated the role of WTA in the phenotypic and functional activation of human monocyte-derived dendritic cells (DCs) treated with ethanol-killed S. aureus. WTA-deficient S. aureus mutant (ΔtagO) exhibited attenuated binding and internalization to DCs compared to the wild-type. ΔtagO induced lower expression of maturation markers on and cytokines in DCs than the wild-type S. aureus. Furthermore, autologous human peripheral blood mononuclear cells cocultured with ΔtagO-treated DCs exhibited a marked reduction in T cell proliferative activity, the expression of activation markers, and the production of cytokines compared to the wild-type S. aureus-stimulated DCs. Collectively, these results suggest that WTA is an important cell wall component of S. aureus for the induction of DC maturation and activation.
Keywords: Dendritic cell; Maturation; Mutant; Staphylococcus aureus; T cell activation; Wall teichoic acid.
Copyright © 2016 Elsevier Ltd. All rights reserved.
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