Interactions of acetylated histones with DNA as revealed by UV laser induced histone-DNA crosslinking - PubMed (original) (raw)
Interactions of acetylated histones with DNA as revealed by UV laser induced histone-DNA crosslinking
Stefanovsky VYu et al. Biochem Biophys Res Commun. 1989.
Abstract
The interaction of acetylated histones with DNA in chromatin has been studied by UV laser-induced crosslinking histones to DNA. After irradiation of the nuclei, the covalently linked protein-DNA complexes were isolated and the presence of histones in them demonstrated immunochemically. When chromatin from irradiated nuclei was treated with clostripain, which selectively cleaved the N-terminal tails of core histones, no one of them was found covalently linked to DNA, thus showing that crosslinking proceeded solely via the N-terminal regions. However, the crosslinking ability of the laser was preserved both upon physiological acetylation of histones, known to be restricted to the N-terminal tails, and with chemically acetylated chromatin. This finding is direct evidence that the postsynthetic histone acetylation does not release the N-terminal tails from interaction with DNA.
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