[3H]muscimol labels neurons in both the superficial and deep layers of cat superior colliculus - PubMed (original) (raw)

Comparative Study

. 1989 Sep 25;104(1-2):31-7.

doi: 10.1016/0304-3940(89)90324-8.

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Comparative Study

[3H]muscimol labels neurons in both the superficial and deep layers of cat superior colliculus

R R Mize et al. Neurosci Lett. 1989.

Abstract

We examined the pattern of [3H]muscimol labeling in cat superior colliculus to determine if it matches that of [3H] gamma-aminobutyric acid ([3H]-GABA) labeling or GABA antibody immunoreactivity. Injections in the superficial layers labeled cell bodies in only the superficial layers. Of 204 labeled cells, 68% were located within the upper 200 microns of the superior colliculus, 31% within the deep superficial gray layer, and only 1% below that layer, a pattern similar to that seen with [3H]GABA labeling. By contrast, an injection in the deep layers of the colliculus resulted in cell labeling in both the superficial and deep layers, including 68% in the superficial gray layer, 24% in the optic layer, and 8% in the intermediate and deep gray layers. This pattern approximates that seen with GABA immunocytochemistry. We conclude that the pattern of accumulation of [3H]muscimol depends critically upon the location of the injection and reasonably matches the pattern of GABA immunoreactivity if the injection involves the deep layers of the colliculus.

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