Pancreatic neoplasia induced by SV40 T-antigen expression in acinar cells of transgenic mice - PubMed (original) (raw)

. 1987 Oct 9;238(4824):188-93.

doi: 10.1126/science.2821617.

Affiliations

• PMID: 2821617
• DOI: 10.1126/science.2821617

Pancreatic neoplasia induced by SV40 T-antigen expression in acinar cells of transgenic mice

D M Ornitz et al. Science. 1987.

Abstract

Three lines of transgenic mice were produced that develop pancreatic neoplasms as a consequence of expression of an elastase I-SV40 T-antigen fusion gene in the acinar cells. A developmental analysis suggests at least a two-stage process in the ontogeny of this disease. The first stage is a T antigen-induced, preneoplastic state characterized by a progression from hyperplasia to dysplasia of the exocrine pancreas, by an increased percentage of tetraploid cells, and by an arrest in acinar cell differentiation. The second stage is characterized by the formation of tumor nodules that appear to be monoclonal, because they have discrete aneuploid DNA contents. The cells within the nodules as compared to normal pancreatic tissue have less total RNA by a factor of 5, less pancreas-specific messenger RNA by a factor of about 50, and increased levels of T-antigen messenger RNA. A tumor cell line has been derived that retains both pancreatic and neoplastic properties.

PubMed Disclaimer

Similar articles

• Formation of the tetraploid intermediate is associated with the development of cells with more than four centrioles in the elastase-simian virus 40 tumor antigen transgenic mouse model of pancreatic cancer.
  Levine DS, Sanchez CA, Rabinovitch PS, Reid BJ. Levine DS, et al. Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6427-31. doi: 10.1073/pnas.88.15.6427. Proc Natl Acad Sci U S A. 1991. PMID: 1650467 Free PMC article.
• Elastase I promoter directs expression of human growth hormone and SV40 T antigen genes to pancreatic acinar cells in transgenic mice.
  Ornitz DM, Palmiter RD, Messing A, Hammer RE, Pinkert CA, Brinster RL. Ornitz DM, et al. Cold Spring Harb Symp Quant Biol. 1985;50:399-409. doi: 10.1101/sqb.1985.050.01.050. Cold Spring Harb Symp Quant Biol. 1985. PMID: 3006998 No abstract available.
• Hyperplasia and tumors of the islets of Langerhans in mice bearing an elastase I-SV40 T-antigen fusion gene.
  Bell RH Jr, Memoli VA, Longnecker DS. Bell RH Jr, et al. Carcinogenesis. 1990 Aug;11(8):1393-8. doi: 10.1093/carcin/11.8.1393. Carcinogenesis. 1990. PMID: 2167183
• SV40 T antigen transgenic mice: cytotoxic T lymphocytes as a selective force in tumor progression.
  Knowles BB, Faas S, Juretic A, Fox N, Crooke R, Hanahan D, Solter D, Jewett L. Knowles BB, et al. Basic Life Sci. 1991;57:111-24; discussion 125. doi: 10.1007/978-1-4684-5994-4_11. Basic Life Sci. 1991. PMID: 1726037 Review. No abstract available.
• Multistep hepatocarcinogenesis in transgenic mice harboring SV40 T-antigen gene.
  Kitagawa T, Hino O, Lee GH, Li H, Liu J, Nomura K, Ohtake K, Furuta Y, Aizawa S. Kitagawa T, et al. Princess Takamatsu Symp. 1991;22:349-60. Princess Takamatsu Symp. 1991. PMID: 1668891 Review.

Cited by

• Multiple endocrine neoplasia induced by the promiscuous expression of a viral oncogene.
  Reynolds RK, Hoekzema GS, Vogel J, Hinrichs SH, Jay G. Reynolds RK, et al. Proc Natl Acad Sci U S A. 1988 May;85(9):3135-9. doi: 10.1073/pnas.85.9.3135. Proc Natl Acad Sci U S A. 1988. PMID: 2452444 Free PMC article.
• Relationship of eukaryotic DNA replication to committed gene expression: general theory for gene control.
  Villarreal LP. Villarreal LP. Microbiol Rev. 1991 Sep;55(3):512-42. doi: 10.1128/mr.55.3.512-542.1991. Microbiol Rev. 1991. PMID: 1943999 Free PMC article. Review.
• Multiple centrosomes arise from tetraploidy checkpoint failure and mitotic centrosome clusters in p53 and RB pocket protein-compromised cells.
  Borel F, Lohez OD, Lacroix FB, Margolis RL. Borel F, et al. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9819-24. doi: 10.1073/pnas.152205299. Epub 2002 Jul 15. Proc Natl Acad Sci U S A. 2002. PMID: 12119403 Free PMC article.
• Animal Models: Challenges and Opportunities to Determine Optimal Experimental Models of Pancreatitis and Pancreatic Cancer.
  Saloman JL, Albers KM, Cruz-Monserrate Z, Davis BM, Edderkaoui M, Eibl G, Epouhe AY, Gedeon JY, Gorelick FS, Grippo PJ, Groblewski GE, Husain SZ, Lai KKY, Pandol SJ, Uc A, Wen L, Whitcomb DC. Saloman JL, et al. Pancreas. 2019 Jul;48(6):759-779. doi: 10.1097/MPA.0000000000001335. Pancreas. 2019. PMID: 31206467 Free PMC article. Review.
• Transgene expression and repression in transgenic rats bearing the phosphoenolpyruvate carboxykinase-simian virus 40 T antigen or the phosphoenolpyruvate carboxykinase-transforming growth factor-alpha constructs.
  Haas MJ, Dragan YP, Hikita H, Shimel R, Takimoto K, Heath S, Vaughan J, Pitot HC. Haas MJ, et al. Am J Pathol. 1999 Jul;155(1):183-92. doi: 10.1016/s0002-9440(10)65112-7. Am J Pathol. 1999. PMID: 10393850 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)