Guanine nucleotide and pyrophosphate activate exogenous phosphatidylinositol 4,5-bisphosphate hydrolysis in rat liver plasma membranes - PubMed (original) (raw)
Guanine nucleotide and pyrophosphate activate exogenous phosphatidylinositol 4,5-bisphosphate hydrolysis in rat liver plasma membranes
D Bojanic et al. Biochem Biophys Res Commun. 1987.
Abstract
5'-guanylylimidodiphosphate (GppNHp) in the presence of deoxycholate, stimulated the phospholipase C-mediated hydrolysis of exogenous [3H]phosphatidylinositol 4,5-bisphosphate ([3H]PIP2) to myo-[3H]inositol 1,4,5-trisphosphate in rat liver plasma membranes. Activation was not specific for guanine nucleotides as 5'-adenylylimidodiphosphate, imidodiphosphate and pyrophosphate stimulated the enzyme with similar efficacies and potencies. Enzyme activation by GppNHp was most pronounced when [3H]PIP2 was used as substrate. No added Ca++ was required for [3H]PIP2 breakdown but hydrolysis was inhibited by divalent ion chelators. GppNHp stimulation was apparent in the presence of Ca++ or Mg++ as well as chelator concentrations that partially inhibited the enzyme, indicating that this effect was not attributed to changes in affinity of these divalent cations for the enzyme or substrate. These results suggest that guanine nucleotides can stimulate the hydrolysis of exogenous [3H]PIP2 in rat liver membranes by a non-specific effect probably due to the interaction of the diphosphate moiety with the enzyme or substrate.
Similar articles
- Assay of a phosphatidylinositol bisphosphate phospholipase C activity in postmortem human brain.
O'Neill C, Fowler CJ, Wiehager B, Alafuzoff I, Winblad B. O'Neill C, et al. Brain Res. 1991 Mar 15;543(2):307-14. doi: 10.1016/0006-8993(91)90042-t. Brain Res. 1991. PMID: 1647835 - Regulation of inositol 1,4,5-trisphosphate metabolism by guanine nucleotides in membranes of cultured newborn rat cardiomyocytes.
Vulliemoz Y, Huber F, Bilezikian JP. Vulliemoz Y, et al. Biochem Pharmacol. 1992 Mar 3;43(5):1001-7. doi: 10.1016/0006-2952(92)90605-i. Biochem Pharmacol. 1992. PMID: 1313233 - Regulation of phosphoinositide breakdown by guanine nucleotides.
Litosch I, Fain JN. Litosch I, et al. Life Sci. 1986 Jul 21;39(3):187-94. doi: 10.1016/0024-3205(86)90529-1. Life Sci. 1986. PMID: 3016436 Review. - Receptor-mediated signalling pathways acting through hydrolysis of membrane phospholipids in cardiomyocytes.
Lamers JM, De Jonge HW, Panagia V, Van Heugten HA. Lamers JM, et al. Cardioscience. 1993 Sep;4(3):121-31. Cardioscience. 1993. PMID: 8400019 Review.
Cited by
- Carbachol and histamine stimulation of guanine-nucleotide-dependent phosphoinositide hydrolysis in rat brain cortical membranes.
Claro E, Garcia A, Picatoste F. Claro E, et al. Biochem J. 1989 Jul 1;261(1):29-35. doi: 10.1042/bj2610029. Biochem J. 1989. PMID: 2549964 Free PMC article. - Phospholipase C-beta 1 is regulated by a pertussis toxin-insensitive G-protein.
Martin TF, Lewis JE, Kowalchyk JA. Martin TF, et al. Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):753-60. doi: 10.1042/bj2800753. Biochem J. 1991. PMID: 1662486 Free PMC article. - Guanine nucleotide- and muscarinic agonist-dependent phosphoinositide metabolism in synaptoneurosomes from cerebral cortex of immature rats.
Candura SM, Castoldi AF, Manzo L, Costa LG. Candura SM, et al. Neurochem Res. 1992 Nov;17(11):1133-41. doi: 10.1007/BF00967291. Neurochem Res. 1992. PMID: 1361027 - Inositol-lipid-specific phospholipase C isoenzymes and their differential regulation by receptors.
Cockcroft S, Thomas GM. Cockcroft S, et al. Biochem J. 1992 Nov 15;288 ( Pt 1)(Pt 1):1-14. doi: 10.1042/bj2880001. Biochem J. 1992. PMID: 1332691 Free PMC article. Review. No abstract available.