Isolation of high-molecular-weight DNA from eukaryotic cells by formamide treatment and dialysis - PubMed (original) (raw)
Isolation of high-molecular-weight DNA from eukaryotic cells by formamide treatment and dialysis
J J Kupiec et al. Anal Biochem. 1987 Jul.
Abstract
A new method for the isolation of high-molecular-weight DNA from eukaryotic cells is presented. It is based on formamide treatment and extensive dialysis of cellular proteinase K digests. This procedure consists mainly of a series of incubations allowing simultaneous preparation of several DNAs which can then be restricted, ligated, hybridized, and cloned.
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