Chemical synthesis of genes for human insulin - PubMed (original) (raw)
Chemical synthesis of genes for human insulin
R Crea et al. Proc Natl Acad Sci U S A. 1978 Dec.
Abstract
A rapid chemical procedure has been developed and used for the synthesis of 29 oligodeoxyribonucleotides to build synthetic genes for human insulin. The gene for insulin B chain, 104 base pairs, and the one for A chain, 77 base pairs, were designed from the amino acid sequence of human polypeptides. They bear single-stranded cohesive termini for the EcoRI and BamHI restriction endonucleases and are designed to be inserted separately into a pBR322 plasmid. The synthetic fragments, deca- to pentadecanucleotides, were synthesized by a block phosphotriester method with trinucleotides as building blocks. Final purification was by high-performance liquid chromatography. All 29 oligonucleotides were pure and had the correct sequences.
Similar articles
- Synthesis of the human insulin gene. Part IV. New synthetic deoxyribooligonucleotide adaptors and primer for DNA cloning and sequence analysis.
Narang SA, Brousseau R, Hsiung HM, Sung W, Scarpulla R, Ghangas G, Lau L, Hess B, Wu R. Narang SA, et al. Nucleic Acids Symp Ser. 1980;(7):377-85. Nucleic Acids Symp Ser. 1980. PMID: 6265876 - Improved phosphotriester method for the synthesis of gene fragments.
Narang SA, Hsiung HM, Brousseau R. Narang SA, et al. Methods Enzymol. 1979;68:90-8. doi: 10.1016/0076-6879(79)68008-4. Methods Enzymol. 1979. PMID: 542130 No abstract available. - Total synthesis of a gene.
Khorana HG. Khorana HG. Science. 1979 Feb 16;203(4381):614-25. doi: 10.1126/science.366749. Science. 1979. PMID: 366749 Review. - [The role of chemical synthesis of DNA in gene technology (author's transl)].
Köster H. Köster H. Arzneimittelforschung. 1980;30(3a):54-57. Arzneimittelforschung. 1980. PMID: 6992790 Review. German.
Cited by
- Pursuing excitonic energy transfer with programmable DNA-based optical breadboards.
Mathur D, Díaz SA, Hildebrandt N, Pensack RD, Yurke B, Biaggne A, Li L, Melinger JS, Ancona MG, Knowlton WB, Medintz IL. Mathur D, et al. Chem Soc Rev. 2023 Nov 13;52(22):7848-7948. doi: 10.1039/d0cs00936a. Chem Soc Rev. 2023. PMID: 37872857 Free PMC article. Review. - Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli.
Karyolaimos A, de Gier JW. Karyolaimos A, et al. Front Bioeng Biotechnol. 2021 Dec 14;9:797334. doi: 10.3389/fbioe.2021.797334. eCollection 2021. Front Bioeng Biotechnol. 2021. PMID: 34970535 Free PMC article. Review. - The discovery of insulin revisited: lessons for the modern era.
Lewis GF, Brubaker PL. Lewis GF, et al. J Clin Invest. 2021 Jan 4;131(1):e142239. doi: 10.1172/JCI142239. J Clin Invest. 2021. PMID: 33393501 Free PMC article. Review. - Making, Cloning, and the Expression of Human Insulin Genes in Bacteria: The Path to Humulin.
Riggs AD. Riggs AD. Endocr Rev. 2021 May 25;42(3):374-380. doi: 10.1210/endrev/bnaa029. Endocr Rev. 2021. PMID: 33340315 Free PMC article. - A new life in a bacterium through synthetic genome: a successful venture by craig venter.
Anand S, Malhotra J, Dua A, Garg N, Saxena A, Sangwan N, Lal D, Verma M, Jindal S, Kaur J, Kumari K, Nigam A, Niharika N, Kaur J, Lal R. Anand S, et al. Indian J Microbiol. 2010 Jun;50(2):125-31. doi: 10.1007/s12088-010-0036-7. Epub 2010 Sep 16. Indian J Microbiol. 2010. PMID: 23100818 Free PMC article. No abstract available.
References
- Nature. 1976 Oct 28;263(5580):748-52 - PubMed
- J Biol Chem. 1975 Jun 25;250(12):4592-600 - PubMed
- Adv Enzymol Relat Areas Mol Biol. 1970;33:445-525 - PubMed
- J Am Chem Soc. 1975 Dec 10;97(25):7327-32 - PubMed
- Nucleic Acids Res. 1974 Mar;1(3):331-53 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources