PEDF improves atherosclerotic plaque stability by inhibiting macrophage inflammation response - PubMed (original) (raw)
PEDF improves atherosclerotic plaque stability by inhibiting macrophage inflammation response
Hao Wen et al. Int J Cardiol. 2017.
Abstract
Background: Atherosclerosis is a vascular disease with plaque formation and growth. Instable plaque with chronic inflammation is closely related to adverse cardiac outcomes. Pigment epithelium-derived factor (PEDF) is an endogenous multifunctional cytokine that possesses the ability of anti-inflammation. The aim of this study is to detect whether PEDF has protective effect on the stability of atherosclerotic plaque and to explore whether the effect of anti-inflammation involved.
Methods and results: ApoE-/- mice fed with high fat diet and RAW264.7 cells were used to evaluate anti-inflammatory activities of PEDF both in vivo and in vitro. PEDF overexpression improved atherosclerotic plaque stability in ApoE-/- mice. The expression of inflammatory factors (interleukin-1β [IL-1β], interleukin-6 [IL-6], tumor necrosis factor-α [TNF-α], monocyte chemotactic protein-1 [MCP-1] and matrix metalloproteinase [MMP-9]) was significantly decreased with PEDF overexpression in vivo and in vitro. The anti-inflammation effect of PEDF was attenuated by PPAR-γ specific antagonist GW9662. In addition, PEDF significantly decreased the expression of phosphorylated ERK-MAPK, p38-MAPK and JNK-MAPK. GW9662 partly reversed the PEDF-mediated depression of phosphorylated ERK- and p38-MAPK but has no significant effect on JNK-MAPK.
Conclusions: PEDF has protective effect on increasing AS plaque stability through ameliorating macrophage inflammation. PPAR-γ and downstream MAPKs were involved in the mechanism.
Keywords: Atherosclerotic plaque stability; Inflammation; MAPK; PEDF; PPAR-γ.
Copyright © 2017. Published by Elsevier B.V.
Comment in
- Does PEDF improve atherosclerotic plaque stability by inhibiting VSMCs' apoptosis?
Luo F, Guo Y, Li X. Luo F, et al. Int J Cardiol. 2017 Sep 1;242:11. doi: 10.1016/j.ijcard.2017.03.038. Int J Cardiol. 2017. PMID: 28619314 No abstract available. - Response to letter "Does PEDF improve atherosclerotic plaque stability by inhibiting VSMCs apoptosis".
Liu M, Wang L, Wen H, Lu H. Liu M, et al. Int J Cardiol. 2017 Sep 1;242:12. doi: 10.1016/j.ijcard.2017.03.158. Int J Cardiol. 2017. PMID: 28619315 No abstract available. - PEDF ameliorates macrophage inflammation via NF-κB suppression.
Ren K, Jiang T, Chen J, Zhao GJ. Ren K, et al. Int J Cardiol. 2017 Nov 15;247:42. doi: 10.1016/j.ijcard.2017.07.069. Int J Cardiol. 2017. PMID: 28916080 No abstract available.
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