Widespread distribution of encapsulin nanocompartments reveals functional diversity - PubMed (original) (raw)
Widespread distribution of encapsulin nanocompartments reveals functional diversity
Tobias W Giessen et al. Nat Microbiol. 2017.
Abstract
Cells organize and regulate their metabolism via membrane- or protein-bound organelles. In this way, incompatible processes can be spatially separated and controlled. In prokaryotes, protein-based compartments are used to sequester harmful reactions and store useful compounds. These protein compartments play key roles in various metabolic and ecological processes, ranging from iron homeostasis to carbon fixation. One of the newest types of protein organelle are encapsulin nanocompartments. They are able to encapsulate specific protein cargo and are proposed to be involved in redox-related processes. We identified more than 900 putative encapsulin systems in bacterial and archaeal genomes. Encapsulins can be found in fifteen bacterial and two archaeal phyla. Our analysis reveals one new capsid type and nine previously unknown cargo proteins targeted to the interior of encapsulins. We experimentally characterize three newly identified encapsulin systems and illustrate their probable involvement in iron mineralization, oxidative and nitrosative stress resistance and anaerobic ammonium oxidation, a process responsible for 30% of the nitrogen lost from the oceans.
Similar articles
- Encapsulins.
Giessen TW. Giessen TW. Annu Rev Biochem. 2022 Jun 21;91:353-380. doi: 10.1146/annurev-biochem-040320-102858. Epub 2022 Mar 18. Annu Rev Biochem. 2022. PMID: 35303791 Free PMC article. Review. - Structure and heterogeneity of a highly cargo-loaded encapsulin shell.
Kwon S, Andreas MP, Giessen TW. Kwon S, et al. J Struct Biol. 2023 Dec;215(4):108022. doi: 10.1016/j.jsb.2023.108022. Epub 2023 Aug 30. J Struct Biol. 2023. PMID: 37657675 - Encapsulins: molecular biology of the shell.
Nichols RJ, Cassidy-Amstutz C, Chaijarasphong T, Savage DF. Nichols RJ, et al. Crit Rev Biochem Mol Biol. 2017 Oct;52(5):583-594. doi: 10.1080/10409238.2017.1337709. Epub 2017 Jun 21. Crit Rev Biochem Mol Biol. 2017. PMID: 28635326 Review. - Advances in encapsulin nanocompartment biology and engineering.
Jones JA, Giessen TW. Jones JA, et al. Biotechnol Bioeng. 2021 Jan;118(1):491-505. doi: 10.1002/bit.27564. Epub 2020 Oct 1. Biotechnol Bioeng. 2021. PMID: 32918485 Free PMC article. Review. - Large-scale computational discovery and analysis of virus-derived microbial nanocompartments.
Andreas MP, Giessen TW. Andreas MP, et al. Nat Commun. 2021 Aug 6;12(1):4748. doi: 10.1038/s41467-021-25071-y. Nat Commun. 2021. PMID: 34362927 Free PMC article.
Cited by
- Structural and biochemical characterization of an encapsulin-associated rhodanese from Acinetobacter baumannii.
Benisch R, Giessen TW. Benisch R, et al. Protein Sci. 2024 Aug;33(8):e5129. doi: 10.1002/pro.5129. Protein Sci. 2024. PMID: 39073218 Free PMC article. - Heterologous Prime-Boost with Immunologically Orthogonal Protein Nanoparticles for Peptide Immunofocusing.
Bhattacharya S, Jenkins MC, Keshavarz-Joud P, Bourque AR, White K, Alvarez Barkane AM, Bryksin AV, Hernandez C, Kopylov M, Finn MG. Bhattacharya S, et al. ACS Nano. 2024 Jul 23;18(31):20083-100. doi: 10.1021/acsnano.4c00949. Online ahead of print. ACS Nano. 2024. PMID: 39041587 Free PMC article. - Myxococcus xanthus encapsulin cargo protein EncD is a flavin-binding protein with ferric reductase activity.
Eren E, Watts NR, Conway JF, Wingfield PT. Eren E, et al. Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2400426121. doi: 10.1073/pnas.2400426121. Epub 2024 May 15. Proc Natl Acad Sci U S A. 2024. PMID: 38748579 - Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages.
Szyszka TN, Andreas MP, Lie F, Miller LM, Adamson LSR, Fatehi F, Twarock R, Draper BE, Jarrold MF, Giessen TW, Lau YH. Szyszka TN, et al. Proc Natl Acad Sci U S A. 2024 May 14;121(20):e2321260121. doi: 10.1073/pnas.2321260121. Epub 2024 May 9. Proc Natl Acad Sci U S A. 2024. PMID: 38722807 - The biosynthesis of the odorant 2-methylisoborneol is compartmentalized inside a protein shell.
Andreas MP, Giessen TW. Andreas MP, et al. bioRxiv [Preprint]. 2024 Apr 23:2024.04.23.590730. doi: 10.1101/2024.04.23.590730. bioRxiv. 2024. PMID: 38712110 Free PMC article. Preprint.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources