Recognition of simian virus 40 T antigen synthesized during viral lytic cycle in monkey kidney cells expressing mouse H-2Kb- and H-2Db-transfected genes by SV40-specific cytotoxic T lymphocytes leads to the abrogation of virus lytic cycle - PubMed (original) (raw)

Recognition of simian virus 40 T antigen synthesized during viral lytic cycle in monkey kidney cells expressing mouse H-2Kb- and H-2Db-transfected genes by SV40-specific cytotoxic T lymphocytes leads to the abrogation of virus lytic cycle

M P Bates et al. Virology. 1988 Jan.

Abstract

Simian virus 40 (SV40)-encoded tumor or T antigen localizes in the membranes in addition to the nucleus of SV40-infected permissive monkey cells and SV40-transformed nonpermissive cells. The surface T antigen in SV40-transformed mouse cells provides a target for the cytotoxic T lymphocytes (CTL) which recognize SV40 T antigen in association with murine K/D, class I H-2 antigens. In order to demonstrate that SV40 T antigen synthesized in SV40-infected permissive monkey kidney cells (TC-7) may also function as a target for CTL, cloned murine H-2Db and H-2Kb genes were expressed in TC-7 cells by DNA transfection and TC-7 cell lines expressing high levels of either H-2Kb or H-Db antigens were established after cell sorting. SV40-infected TC-7/H-2Kb and TC-7/H-2Db cells became susceptible to lysis by SV40-specific H-2b restricted CTL. The susceptibility of these transfected SV40-infected monkey cells to anti-SV40 bulk culture CTL and SV40-specific H-2Db- and H-2Db-restricted CTL clones depended upon the synthesis of SV40 T antigen and the expression of the appropriate H-2Kb or H-2Db restriction elements. Treatment of SV40-infected TC-7/H-2Db and TC-7/H-2Kb with CTL clones abrogated the virus lytic cycle indicating that CTL may play an important role in limiting papovavirus infection in the natural host.

PubMed Disclaimer

Cited by

Cul7/p185/p193 binding to simian virus 40 large T antigen has a role in cellular transformation.
Ali SH, Kasper JS, Arai T, DeCaprio JA. Ali SH, et al. J Virol. 2004 Mar;78(6):2749-57. doi: 10.1128/jvi.78.6.2749-2757.2004. J Virol. 2004. PMID: 14990695 Free PMC article.
Cross-reactive lysis of human targets infected with prototypic and clinical human immunodeficiency virus type 1 (HIV-1) strains by murine anti-HIV-1 IIIB env-specific cytotoxic T lymphocytes.
Chada S, DeJesus CE, Townsend K, Lee WT, Laube L, Jolly DJ, Chang SM, Warner JF. Chada S, et al. J Virol. 1993 Jun;67(6):3409-17. doi: 10.1128/JVI.67.6.3409-3417.1993. J Virol. 1993. PMID: 8497058 Free PMC article.
Class I major histocompatibility proteins as cell surface receptors for simian virus 40.
Atwood WJ, Norkin LC. Atwood WJ, et al. J Virol. 1989 Oct;63(10):4474-7. doi: 10.1128/JVI.63.10.4474-4477.1989. J Virol. 1989. PMID: 2476575 Free PMC article.
Dissection of H-2Db-restricted cytotoxic T-lymphocyte epitopes on simian virus 40 T antigen by the use of synthetic peptides and H-2Dbm mutants.
Tevethia SS, Lewis M, Tanaka Y, Milici J, Knowles B, Maloy WL, Anderson R. Tevethia SS, et al. J Virol. 1990 Mar;64(3):1192-200. doi: 10.1128/JVI.64.3.1192-1200.1990. J Virol. 1990. PMID: 1689391 Free PMC article.

Recognition of simian virus 40 T antigen synthesized during viral lytic cycle in monkey kidney cells expressing mouse H-2Kb- and H-2Db-transfected genes by SV40-specific cytotoxic T lymphocytes leads to the abrogation of virus lytic cycle - PubMed (original) (raw)