Cell division and death in the mouse blastocyst before implantation - PubMed (original) (raw)
. 1986 Oct;195(8):519-526.
doi: 10.1007/BF00375893.
Affiliations
- PMID: 28305692
- DOI: 10.1007/BF00375893
Cell division and death in the mouse blastocyst before implantation
Alan H Handyside et al. Rouxs Arch Dev Biol. 1986 Oct.
Abstract
The numbers of cells in the trophectoderm (TE) and inner cell mass (ICM) of mouse blastocysts were counted by differentially labelling their nuclei with two polynucleotide-specific fluorochromes. Blastocysts recovered from the uterus at intervals between their formation early on Day 4 to the initial stages of implantation on day 5 were analysed. TE cell number increase was initially rapid, indicating some synchronisation of the sixth division, but slowed down progressively and plateaued on Day 5, possibly due to the onset of primary giant cell formation. ICM cell number increase was slower than the corresponding TE cells. As a result, TE cell number more than quadrupled, whereas ICM cell number only doubled over this period. Although the mitotic index of both populations of cells fell steadily, there was no significant difference between them. The decline in the proportion of ICM cells, therefore, is likely to be due to cell death, first detected in early blastocysts and predominantly located in the ICM. In addition, however, a contribution of ICM cells to the overlying polar TE cannot be excluded.
Keywords: Cell death; Cleavage; Inner cell mass; Mouse blastocyst; Trophectoderm.
Similar articles
- The human blastocyst: cell number, death and allocation during late preimplantation development in vitro.
Hardy K, Handyside AH, Winston RM. Hardy K, et al. Development. 1989 Nov;107(3):597-604. doi: 10.1242/dev.107.3.597. Development. 1989. PMID: 2612378 - Decreased inner cell mass proportion in blastocysts from diabetic rats.
Pampfer S, de Hertogh R, Vanderheyden I, Michiels B, Vercheval M. Pampfer S, et al. Diabetes. 1990 Apr;39(4):471-6. doi: 10.2337/diab.39.4.471. Diabetes. 1990. PMID: 2318348 - Making the first decision: lessons from the mouse.
Jedrusik A. Jedrusik A. Reprod Med Biol. 2015 Apr 16;14(4):135-150. doi: 10.1007/s12522-015-0206-8. eCollection 2015 Oct. Reprod Med Biol. 2015. PMID: 29259411 Free PMC article. Review.
Cited by
- From contemplation to classification of chromosomal mosaicism in human preimplantation embryos.
Lebedev IN, Zhigalina DI. Lebedev IN, et al. J Assist Reprod Genet. 2021 Nov;38(11):2833-2848. doi: 10.1007/s10815-021-02304-z. Epub 2021 Sep 13. J Assist Reprod Genet. 2021. PMID: 34518954 Free PMC article. Review. - Four simple rules that are sufficient to generate the mammalian blastocyst.
Nissen SB, Perera M, Gonzalez JM, Morgani SM, Jensen MH, Sneppen K, Brickman JM, Trusina A. Nissen SB, et al. PLoS Biol. 2017 Jul 12;15(7):e2000737. doi: 10.1371/journal.pbio.2000737. eCollection 2017 Jul. PLoS Biol. 2017. PMID: 28700688 Free PMC article. - Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo.
Morris SA, Teo RT, Li H, Robson P, Glover DM, Zernicka-Goetz M. Morris SA, et al. Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6364-9. doi: 10.1073/pnas.0915063107. Epub 2010 Mar 22. Proc Natl Acad Sci U S A. 2010. PMID: 20308546 Free PMC article. - A comparison of polarized and non-polarized human endometrial monolayer culture systems on murine embryo development.
Baghaban Eslami Nejad MR, Rezazadeh Valojerdi M, Kazemi Ashtiani S. Baghaban Eslami Nejad MR, et al. J Exp Clin Assist Reprod. 2005 Apr 19;2(1):7. doi: 10.1186/1743-1050-2-7. J Exp Clin Assist Reprod. 2005. PMID: 15840171 Free PMC article. - Active cell movements coupled to positional induction are involved in lineage segregation in the mouse blastocyst.
Meilhac SM, Adams RJ, Morris SA, Danckaert A, Le Garrec JF, Zernicka-Goetz M. Meilhac SM, et al. Dev Biol. 2009 Jul 15;331(2):210-21. doi: 10.1016/j.ydbio.2009.04.036. Epub 2009 May 5. Dev Biol. 2009. PMID: 19422818 Free PMC article.
References
- Int Rev Cytol. 1980;68:251-306 - PubMed
- J Embryol Exp Morphol. 1978 Dec;48:109-25 - PubMed
- J Embryol Exp Morphol. 1980 Aug;58:231-49 - PubMed
- J Embryol Exp Morphol. 1970 Jun;23(3):693-704 - PubMed
- Nature. 1980 Apr 10;284(5756):555-6 - PubMed
LinkOut - more resources
Research Materials