Tat protein from human immunodeficiency virus forms a metal-linked dimer - PubMed (original) (raw)
Comparative Study
. 1988 Apr 1;240(4848):70-3.
doi: 10.1126/science.2832944.
Affiliations
- PMID: 2832944
- DOI: 10.1126/science.2832944
Comparative Study
Tat protein from human immunodeficiency virus forms a metal-linked dimer
A D Frankel et al. Science. 1988.
Abstract
Tat, the transactivating protein from HIV, forms a metal-linked dimer with metal ions bridging cysteine-rich regions from each monomer. This novel arrangement is distinct from the "zinc finger" domain observed in other eukaryotic regulatory proteins. Ultraviolet absorption spectra show that Tat binds two Zn2+ or two Cd2+ ions per monomer, and electrophoresis of the Tat-metal complexes demonstrates that the protein forms metal-linked dimers. Partial proteolysis and circular dichroism spectra suggest that metal binding has its primary effects in the cysteine-rich region and relatively little effect on the folding of other regions. These results suggest new directions for biological studies and new approaches to drug design.
Similar articles
- Dimerization of the tat protein from human immunodeficiency virus: a cysteine-rich peptide mimics the normal metal-linked dimer interface.
Frankel AD, Chen L, Cotter RJ, Pabo CO. Frankel AD, et al. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6297-300. doi: 10.1073/pnas.85.17.6297. Proc Natl Acad Sci U S A. 1988. PMID: 2842763 Free PMC article. - Structural characterization of the metal binding site in the cysteine-rich region of HIV-1 Tat protein.
Huang HW, Wang KT. Huang HW, et al. Biochem Biophys Res Commun. 1996 Oct 14;227(2):615-21. doi: 10.1006/bbrc.1996.1554. Biochem Biophys Res Commun. 1996. PMID: 8878561 - Activity of synthetic tat peptides in human immunodeficiency virus type 1 long terminal repeat-promoted transcription in a cell-free system.
Jeyapaul J, Reddy MR, Khan SA. Jeyapaul J, et al. Proc Natl Acad Sci U S A. 1990 Sep;87(18):7030-4. doi: 10.1073/pnas.87.18.7030. Proc Natl Acad Sci U S A. 1990. PMID: 2205850 Free PMC article. - Zn2+ binding to cysteine-rich domain of extracellular human immunodeficiency virus type 1 Tat protein is associated with Tat protein-induced apoptosis.
Misumi S, Takamune N, Ohtsubo Y, Waniguchi K, Shoji S. Misumi S, et al. AIDS Res Hum Retroviruses. 2004 Mar;20(3):297-304. doi: 10.1089/088922204322996536. AIDS Res Hum Retroviruses. 2004. PMID: 15117453
Cited by
- Bioinformatics Insights on Viral Gene Expression Transactivation: From HIV-1 to SARS-CoV-2.
Patarca R, Haseltine WA. Patarca R, et al. Int J Mol Sci. 2024 Mar 16;25(6):3378. doi: 10.3390/ijms25063378. Int J Mol Sci. 2024. PMID: 38542351 Free PMC article. - Advancement and application of novel cell-penetrating peptide in cancer management.
Yadav S, Singh P. Yadav S, et al. 3 Biotech. 2023 Jul;13(7):234. doi: 10.1007/s13205-023-03649-1. Epub 2023 Jun 13. 3 Biotech. 2023. PMID: 37323859 Free PMC article. Review. - HIV-1 Tat and Heparan Sulfate Proteoglycans Orchestrate the Setup of in Cis and in Trans Cell-Surface Interactions Functional to Lymphocyte Trans-Endothelial Migration.
Urbinati C, Milanesi M, Lauro N, Bertelli C, David G, D'Ursi P, Rusnati M, Chiodelli P. Urbinati C, et al. Molecules. 2021 Dec 10;26(24):7488. doi: 10.3390/molecules26247488. Molecules. 2021. PMID: 34946571 Free PMC article. - Endolysosome iron restricts Tat-mediated HIV-1 LTR transactivation by increasing HIV-1 Tat oligomerization and β-catenin expression.
Khan N, Halcrow PW, Lakpa LK, Rehan M, Chen X, Geiger JD. Khan N, et al. J Neurovirol. 2021 Oct;27(5):755-773. doi: 10.1007/s13365-021-01016-5. Epub 2021 Sep 22. J Neurovirol. 2021. PMID: 34550543 Free PMC article. - Tat-CIAPIN1 protein prevents against cytokine-induced cytotoxicity in pancreatic RINm5F β-cells.
Yeo HJ, Shin MJ, Kim DW, Kwon HY, Eum WS, Choi SY. Yeo HJ, et al. BMB Rep. 2021 Sep;54(9):458-463. doi: 10.5483/BMBRep.2021.54.9.040. BMB Rep. 2021. PMID: 34120676 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources