Purification of 120,000 dalton envelope glycoprotein from culture fluids of human immunodeficiency virus (HIV)-infected H9 cells - PubMed (original) (raw)
Purification of 120,000 dalton envelope glycoprotein from culture fluids of human immunodeficiency virus (HIV)-infected H9 cells
S W Pyle et al. AIDS Res Hum Retroviruses. 1987.
Abstract
The outer envelope glycoprotein, gp120, has been purified from large volumes (greater than 100 liters) of HTLV-IIIB-infected H9 cell culture fluids using immunoaffinity chromatography resins prepared from immunoglobulins of AIDS patients plasma. By using a single-step immunoaffinity purification, between 7 and 28 micrograms of gp120 was recovered from each liter of culture fluid which represented between 60 and 95% of the total envelope glycoprotein present in the fluid. Envelope glycoprotein in culture media was concentrated more than 10,000 times over the starting material. The water-soluble gp120, containing trace contaminating proteins, was purified to apparent homogeneity by preparative polyacrylamide gel electrophoresis (PAGE). Envelope glycoprotein purified from culture fluids was immunogenic in laboratory animals in both native and PAGE-purified forms and was reactive with AIDS patient sera in immunoassays.
Similar articles
- Rapid assessment of relationships among HIV isolates by oligopeptide analyses of external envelope glycoproteins.
Robey WG, Nara PL, Poore CM, Popovic M, McLane MF, Barin F, Essex M, Fischinger PJ. Robey WG, et al. AIDS Res Hum Retroviruses. 1987;3(4):401-8. doi: 10.1089/aid.1987.3.401. AIDS Res Hum Retroviruses. 1987. PMID: 2833916 - Purification and characterization of the external envelope glycoprotein from two human immunodeficiency virus type 1 variants, HTLV-IIIB and HTLV-IIIRF.
Pyle SW, Dubois GC, Robey WG, Bess JW Jr, Fischinger PJ, Arthur LO. Pyle SW, et al. J Virol. 1988 Jul;62(7):2258-64. doi: 10.1128/JVI.62.7.2258-2264.1988. J Virol. 1988. PMID: 3163739 Free PMC article. - Partial purification of native HIV transmembrane protein gp41: generation of polyclonal and monoclonal antibodies.
Zweig M, Showalter SD, Bladen SV, Gilden RV, Arthur LO, Robey WG, Nara PL, Fischinger PJ. Zweig M, et al. AIDS Res Hum Retroviruses. 1988 Feb;4(1):51-62. doi: 10.1089/aid.1988.4.51. AIDS Res Hum Retroviruses. 1988. PMID: 3259140 - Characterization of CD4 glycoprotein determinant-HIV envelope protein interactions: perspectives for analog and vaccine development.
Farrar WL, Harel-Bellan A, Ferris DK. Farrar WL, et al. Crit Rev Immunol. 1988;8(4):315-39. Crit Rev Immunol. 1988. PMID: 2850890 Review.
Cited by
- Efficiently cleaved HIV-1 envelopes: can they be important for vaccine immunogen development?
Das S, Kumar R, Ahmed S, Parray HA, Samal S. Das S, et al. Ther Adv Vaccines Immunother. 2020 Oct 8;8:2515135520957763. doi: 10.1177/2515135520957763. eCollection 2020. Ther Adv Vaccines Immunother. 2020. PMID: 33103053 Free PMC article. Review. - HIV Apheresis Tags (HIVAT) Aided Elimination of Viremia.
Malecki M, Saetre B. Malecki M, et al. Mol Cell Ther. 2018;6(1):6. doi: 10.26781/2052-8426-2018-06. Epub 2018 Jun 21. Mol Cell Ther. 2018. PMID: 30931130 Free PMC article. - Increased, Durable B-Cell and ADCC Responses Associated with T-Helper Cell Responses to HIV-1 Envelope in Macaques Vaccinated with gp140 Occluded at the CD4 Receptor Binding Site.
Bogers WMJM, Barnett SW, Oostermeijer H, Nieuwenhuis IG, Beenhakker N, Mortier D, Mooij P, Koopman G, Remarque E, Martin G, Lai RP, Dey AK, Sun Y, Burke B, Ferrari G, Montefiori D, Martin L, Davis D, Srivastava I, Heeney JL. Bogers WMJM, et al. J Virol. 2017 Sep 12;91(19):e00811-17. doi: 10.1128/JVI.00811-17. Print 2017 Oct 1. J Virol. 2017. PMID: 28701402 Free PMC article. - HIV gp120 binds to mannose receptor on vaginal epithelial cells and induces production of matrix metalloproteinases.
Fanibunda SE, Modi DN, Gokral JS, Bandivdekar AH. Fanibunda SE, et al. PLoS One. 2011;6(11):e28014. doi: 10.1371/journal.pone.0028014. Epub 2011 Nov 22. PLoS One. 2011. PMID: 22132194 Free PMC article. - Envelope glycoprotein incorporation, not shedding of surface envelope glycoprotein (gp120/SU), Is the primary determinant of SU content of purified human immunodeficiency virus type 1 and simian immunodeficiency virus.
Chertova E, Bess JW Jr, Crise BJ, Sowder II RC, Schaden TM, Hilburn JM, Hoxie JA, Benveniste RE, Lifson JD, Henderson LE, Arthur LO. Chertova E, et al. J Virol. 2002 Jun;76(11):5315-25. doi: 10.1128/jvi.76.11.5315-5325.2002. J Virol. 2002. PMID: 11991960 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources