Orientation of the cytoplasmically made subunits of beef heart cytochrome c oxidase determined by protease digestion and antibody binding experiments - PubMed (original) (raw)
. 1988 Feb 23;27(4):1389-94.
doi: 10.1021/bi00404a045.
Affiliations
- PMID: 2835091
- DOI: 10.1021/bi00404a045
Orientation of the cytoplasmically made subunits of beef heart cytochrome c oxidase determined by protease digestion and antibody binding experiments
Y Z Zhang et al. Biochemistry. 1988.
Abstract
The topology of several of the cytoplasmically made subunits of beef heart cytochrome c oxidase has been determined by protease digestion of oriented membrane preparations, using subunit-specific antibodies to identify cleavage products. Reconstituted vesicles of cytochrome c oxidase and asolectin were used as a vesicle preparation with the C domain of the enzyme available for protease digestion. Submitochondrial particles were used as vesicles with the M domain outermost. Trypsin and/or proteinase K cleaved polypeptides CIV, ASA, AED, STA, and IHQ. Cleavage of CIV, STA, and IHQ was from the M domains only and involved the removal of a fragment from the N-terminus in each case. Polypeptide AED was cleaved from the C side in the N-terminal part, while ASA was cleaved from both the C and M domains. Polypeptide fragments were electroblotted from polyacrylamide gels onto derivatized glass paper and sites of proteolytic cleavage determined by N-terminal sequence analysis.
Similar articles
- Topology of subunits of the mammalian cytochrome c oxidase: relationship to the assembly of the enzyme complex.
Zhang YZ, Ewart G, Capaldi RA. Zhang YZ, et al. Biochemistry. 1991 Apr 16;30(15):3674-81. doi: 10.1021/bi00229a012. Biochemistry. 1991. PMID: 1707666 - Arrangement of subunit IV in beef heart cytochrome c oxidase probed by chemical labeling and protease digestion experiments.
Malatesta F, Darley-Usmar V, de Jong C, Prochaska LJ, Bisson R, Capaldi RA, Steffens GC, Buse G. Malatesta F, et al. Biochemistry. 1983 Sep 13;22(19):4405-11. doi: 10.1021/bi00288a010. Biochemistry. 1983. PMID: 6313039 - Topology of beef heart cytochrome c oxidase from studies on reconstituted membranes.
Zhang YZ, Georgevich G, Capaldi RA. Zhang YZ, et al. Biochemistry. 1984 Nov 6;23(23):5616-21. doi: 10.1021/bi00318a036. Biochemistry. 1984. PMID: 6095902 - Catalytic activity and arrangement of subunit polypeptides in rat liver cytochrome c oxidase as studied by proteolysis.
Nagasawa-Fujimori H, Nagasawa T, Heinrich PC. Nagasawa-Fujimori H, et al. Biochim Biophys Acta. 1980 Feb 8;589(2):336-45. doi: 10.1016/0005-2728(80)90049-3. Biochim Biophys Acta. 1980. PMID: 6243969 - Subunit arrangement in beef heart complex III.
González-Halphen D, Lindorfer MA, Capaldi RA. González-Halphen D, et al. Biochemistry. 1988 Sep 6;27(18):7021-31. doi: 10.1021/bi00418a053. Biochemistry. 1988. PMID: 2848575
Cited by
- Tissue-specific regulation of bovine heart cytochrome-c oxidase activity by ADP via interaction with subunit VIa.
Anthony G, Reimann A, Kadenbach B. Anthony G, et al. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1652-6. doi: 10.1073/pnas.90.5.1652. Proc Natl Acad Sci U S A. 1993. PMID: 8383320 Free PMC article. - Complexity and tissue specificity of the mitochondrial respiratory chain.
Capaldi RA, Halphen DG, Zhang YZ, Yanamura W. Capaldi RA, et al. J Bioenerg Biomembr. 1988 Jun;20(3):291-311. doi: 10.1007/BF00769634. J Bioenerg Biomembr. 1988. PMID: 2841307 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources