Stimulation of EBV-activated human B cells by monocytes and monocyte products. Role of IFN-beta 2/B cell stimulatory factor 2/IL-6 - PubMed (original) (raw)

. 1988 Jun 15;140(12):4329-36.

Affiliations

• PMID: 2836512

Stimulation of EBV-activated human B cells by monocytes and monocyte products. Role of IFN-beta 2/B cell stimulatory factor 2/IL-6

G Tosato et al. J Immunol. 1988.

Abstract

EBV infects human B lymphocytes and induces them to proliferate, to produce Ig, and to give rise to immortal cell lines. Although the mechanisms of B cell activation by EBV are largely unknown, the continuous proliferation of EBV-immortalized B cells is dependent, at least in part, upon autocrine growth factors produced by the same EBV-infected B cells. In the present studies we have examined the influence of monocytes on B cell activation by EBV and found that unlike peripheral blood T cells and B cells, monocytes enhance by as much as 30- to 50-fold virus-induced B cell proliferation and Ig production. Upon activation with LPS, monocytes secrete a growth factor activity that promotes both proliferation and Ig secretion in EBV-infected B cells and thus reproduces the effects of monocytes in these cultures. Unlike a number of other factors, rIFN-beta 2/B cell stimulatory factor 2 (BSF-2)/IL-6 stimulates the growth of human B cells activated by EBV in a manner similar to that induced by activated monocyte supernatants. In addition, an antiserum to IFN-beta that recognizes both IFN-beta 1 and IFN-beta 2 completely neutralizes the B cell growth factor activity of activated monocyte supernatants. These findings demonstrate that IFN-beta 2/BSF-2/IL-6 is a growth factor for human B cells activated by EBV and suggest that this molecule is responsible for B cell growth stimulation induced by activated monocyte supernatants. We have examined the possibility that IFN-beta 2/BSF-2/IL-6 might also be responsible for B cell growth stimulation by supernatants of EBV-immortalized B cells and thus may function as an autocrine growth factor. However, IFN-beta 2/BSF-2/IL-6 is not detectable in supernatants of EBV-immortalized B cells by immunoprecipitation. Also, an antiserum to IFN-beta that neutralizes IFN-beta 2/BSF-2/IL-6 fails to neutralize autocrine growth factor activity. This suggests that autocrine growth factors produced by EBV-immortalized B cells are distinct from IFN-beta 2/BSF-2/IL-6. Thus, the continuous proliferation of EBV-immortalized B cells is enhanced by either autocrine or paracrine growth factors. One of the mediators with paracrine growth factor activity is IFN-beta 2/BSF-2/IL-6.

PubMed Disclaimer

Similar articles

• Epstein-Barr virus-immortalized B cells produce IL-6 as an autocrine growth factor.
  Yokoi T, Miyawaki T, Yachie A, Kato K, Kasahara Y, Taniguchi N. Yokoi T, et al. Immunology. 1990 May;70(1):100-5. Immunology. 1990. PMID: 2162322 Free PMC article.
• Antigen presentation by EBV-B cells to resting and activated T cells: role of interleukin 1.
  Chu ET, Lareau M, Rosenwasser LJ, Dinarello CA, Geha RS. Chu ET, et al. J Immunol. 1985 Mar;134(3):1676-81. J Immunol. 1985. PMID: 2981918
• Interactions involving cyclosporine A, interleukin-6, and Epstein-Barr virus lead to the promotion of B-cell lymphoproliferative disease.
  Tanner JE, Alfieri C. Tanner JE, et al. Leuk Lymphoma. 1996 May;21(5-6):379-90. doi: 10.3109/10428199609093435. Leuk Lymphoma. 1996. PMID: 9172802 Review.
• The cloning and biological characterization of recombinant human interleukin 11.
  Paul SR, Schendel P. Paul SR, et al. Int J Cell Cloning. 1992 May;10(3):135-43. doi: 10.1002/stem.5530100303. Int J Cell Cloning. 1992. PMID: 1535352 Review.

Cited by

• Human plasma accelerates immortalization of B lymphocytes by Epstein-Barr virus.
  Manor E. Manor E. Cell Prolif. 2008 Apr;41(2):292-8. doi: 10.1111/j.1365-2184.2008.00513.x. Cell Prolif. 2008. PMID: 18336473 Free PMC article.
• The antiviral prophylaxis of post-transplant lymphoproliferative disorder.
  Davis CL. Davis CL. Springer Semin Immunopathol. 1998;20(3-4):437-53. doi: 10.1007/BF00838054. Springer Semin Immunopathol. 1998. PMID: 9870256 Review. No abstract available.
• Post-transplant lymphoproliferative disease (PTLD): lymphokine production and PTLD.
  Tosato G, Teruya-Feldstein J, Setsuda J, Pike SE, Jones KD, Jaffe ES. Tosato G, et al. Springer Semin Immunopathol. 1998;20(3-4):405-23. doi: 10.1007/BF00838052. Springer Semin Immunopathol. 1998. PMID: 9870254 Review. No abstract available.
• Vasostatin, a calreticulin fragment, inhibits angiogenesis and suppresses tumor growth.
  Pike SE, Yao L, Jones KD, Cherney B, Appella E, Sakaguchi K, Nakhasi H, Teruya-Feldstein J, Wirth P, Gupta G, Tosato G. Pike SE, et al. J Exp Med. 1998 Dec 21;188(12):2349-56. doi: 10.1084/jem.188.12.2349. J Exp Med. 1998. PMID: 9858521 Free PMC article.
• Inactivation of the integrin beta 6 subunit gene reveals a role of epithelial integrins in regulating inflammation in the lung and skin.
  Huang XZ, Wu JF, Cass D, Erle DJ, Corry D, Young SG, Farese RV Jr, Sheppard D. Huang XZ, et al. J Cell Biol. 1996 May;133(4):921-8. doi: 10.1083/jcb.133.4.921. J Cell Biol. 1996. PMID: 8666675 Free PMC article.

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations