Characterization of the promoter for the alpha 1 (IV) collagen gene. DNA sequences within the first intron enhance transcription - PubMed (original) (raw)

. 1988 Sep 5;263(25):12310-4.

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Characterization of the promoter for the alpha 1 (IV) collagen gene. DNA sequences within the first intron enhance transcription

P D Killen et al. J Biol Chem. 1988.

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Abstract

Two overlapping clones spanning 19 kilobase pairs (kb) of the 5' end of the alpha 1 (IV) collagen gene were isolated and found to contain a single exon which encoded the 5'-untranslated sequence and 84 base pairs of the signal peptide. The 5' end of this exon was determined to be the 5' end of the transcript by S1 nuclease protection and primer extension. The nucleotide sequence of 1 kb of the 5'-flanking DNA was extremely G + C-rich (greater than 70%) and contained two GC boxes and a putative cAMP regulatory sequence. The transcriptional regulation of the alpha 1 (IV) gene was studied with chimeric gene constructs utilizing 2.5 kb of the 5'-flanking sequence coupled to the gene for chloramphenicol acetyltransferase. Transfection of this construct into differentiating F9 cells resulted in low chloramphenicol acetyltransferase activity compared to beta-actin or Rous sarcoma virus long terminal repeat promoters, although these cells produce large amounts of collagen IV. Inclusion of a 2.7-kb sequence 2.3 kb downstream from the first exon in either orientation increased the transcription of the chloramphenicol acetyltransferase construct approximately 10-fold in F9 cells, but was not active in NIH 3T3 cells, which synthesize little collagen IV. These results indicate the presence of an enhancer within the first intron, which increases the expression of this gene.

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