Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein - PubMed (original) (raw)
Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein
C di Guan et al. Gene. 1988.
Abstract
Vectors were constructed that allow foreign peptides to be expressed in Escherichia coli as fusion proteins. The peptides are fused to the C terminus of maltose-binding protein (MBP), which allows them to be purified by the MBP's affinity to cross-linked amylose (starch). The fusion protein can be directed to the periplasm by including the leader sequence from the phoA gene on the vector.
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