The expression of Helicobacter pylori tfs plasticity zone cluster is regulated by pH and adherence, and its composition is associated with differential gastric IL-8 secretion - PubMed (original) (raw)
doi: 10.1111/hel.12390. Epub 2017 Apr 24.
Affiliations
- PMID: 28436598
- DOI: 10.1111/hel.12390
The expression of Helicobacter pylori tfs plasticity zone cluster is regulated by pH and adherence, and its composition is associated with differential gastric IL-8 secretion
Bruno Silva et al. Helicobacter. 2017 Aug.
Abstract
Background: Helicobacter pylori virulence is associated with different clinical outcomes. The existence of an intact dupA gene from tfs4b cluster has been suggested as a predictor for duodenal ulcer development. However, the role of tfs plasticity zone clusters in the development of ulcers remains unclear. We studied several H. pylori strains to characterize the gene arrangement of tfs3 and tfs4 clusters and their impact in the inflammatory response by infected gastric cells.
Methods: The genome of 14 H. pylori strains isolated from Western patients, pediatric (n=10) and adult (n=4), was fully sequenced using the Illumina platform MiSeq, in addition to eight pediatric strains previously sequenced. These strains were used to infect human gastric cells, and the secreted interleukin-8 (IL-8) was quantified by ELISA. The expression of virB2, dupA, virB8, virB10, and virB6 was assessed by quantitative PCR in adherent and nonadherent fractions of H. pylori during in vitro co-infection, at different pH values.
Results: We have found that cagA-positive H. pylori strains harboring a complete tfs plasticity zone cluster significantly induce increased production of IL-8 from gastric cells. We have also found that the region spanning from virB2 to virB10 genes constitutes an operon, whose expression is increased in the adherent fraction of bacteria during infection, as well as in both adherent and nonadherent fractions at acidic conditions.
Conclusions: A complete tfs plasticity zone cluster is a virulence factor that may be important for the colonization of H. pylori and to the development of severe outcomes of the infection with cagA-positive strains.
Keywords: Helicobacter pylori; adhesion; complete tfs cluster; interleukin-8; operon; pH.
© 2017 John Wiley & Sons Ltd.
Similar articles
- Helicobacter pylori with the Intact dupA Cluster is more Virulent than the Strains with the Incomplete dupA Cluster.
Wang MY, Shao C, Li J, Yang YC, Wang SB, Hao JL, Wu CM, Gao XZ, Shao SH. Wang MY, et al. Curr Microbiol. 2015 Jul;71(1):16-23. doi: 10.1007/s00284-015-0812-z. Epub 2015 Apr 7. Curr Microbiol. 2015. PMID: 25847580 - Patterns of Adherence of Helicobacter pylori Clinical Isolates to Epithelial Cells, and its Association with Disease and with Virulence Factors.
Vázquez-Jiménez FE, Torres J, Flores-Luna L, Cerezo SG, Camorlinga-Ponce M. Vázquez-Jiménez FE, et al. Helicobacter. 2016 Feb;21(1):60-8. doi: 10.1111/hel.12230. Epub 2015 Apr 24. Helicobacter. 2016. PMID: 25908566 - The intact dupA cluster is a more reliable Helicobacter pylori virulence marker than dupA alone.
Jung SW, Sugimoto M, Shiota S, Graham DY, Yamaoka Y. Jung SW, et al. Infect Immun. 2012 Jan;80(1):381-7. doi: 10.1128/IAI.05472-11. Epub 2011 Oct 28. Infect Immun. 2012. PMID: 22038914 Free PMC article. - Mucosal interleukin-8 and Helicobacter pylori-associated gastroduodenal disease.
Crabtree JE, Lindley IJ. Crabtree JE, et al. Eur J Gastroenterol Hepatol. 1994 Dec;6 Suppl 1:S33-8. Eur J Gastroenterol Hepatol. 1994. PMID: 7735932 Review. - Helicobacter pylori: molecular basis for colonization and survival in gastric environment and resistance to antibiotics. A short review.
Fagoonee S, Pellicano R. Fagoonee S, et al. Infect Dis (Lond). 2019 Jun;51(6):399-408. doi: 10.1080/23744235.2019.1588472. Epub 2019 Mar 25. Infect Dis (Lond). 2019. PMID: 30907202 Review.
Cited by
- Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient.
Phuc BH, Tuan VP, Binh TT, Tung PH, Tri TD, Dung HDQ, Thuan NPM, Fauzia KA, Tshibangu-Kabamba E, Alfaray RI, Saruuljavkhlan B, Matsumoto T, Akada J, Yamaoka Y. Phuc BH, et al. Sci Rep. 2023 May 31;13(1):8869. doi: 10.1038/s41598-023-35527-4. Sci Rep. 2023. PMID: 37258611 Free PMC article. - Gene content, phage cycle regulation model and prophage inactivation disclosed by prophage genomics in the Helicobacter pylori Genome Project.
Vale FF, Roberts RJ, Kobayashi I, Camargo MC, Rabkin CS; HpGP Research Network. Vale FF, et al. Gut Microbes. 2024 Jan-Dec;16(1):2379440. doi: 10.1080/19490976.2024.2379440. Epub 2024 Aug 12. Gut Microbes. 2024. PMID: 39132840 Free PMC article. - Helicobacter pylori infection and gastric cancer biology: tempering a double-edged sword.
Mentis AA, Boziki M, Grigoriadis N, Papavassiliou AG. Mentis AA, et al. Cell Mol Life Sci. 2019 Jul;76(13):2477-2486. doi: 10.1007/s00018-019-03044-1. Epub 2019 Feb 19. Cell Mol Life Sci. 2019. PMID: 30783683 Free PMC article. - Overview of Helicobacter pylori Infection in Indonesia: What Distinguishes It from Countries with High Gastric Cancer Incidence?
Miftahussurur M, Waskito LA, Fauzia KA, Mahmudah I, Doohan D, Adnyana IK, Khomsan A, Ratnasari N, Rezkitha YAA. Miftahussurur M, et al. Gut Liver. 2021 Sep 15;15(5):653-665. doi: 10.5009/gnl20019. Gut Liver. 2021. PMID: 32616679 Free PMC article. Review. - Biomarker Characterization and Prediction of Virulence and Antibiotic Resistance from Helicobacter pylori Next Generation Sequencing Data.
Vital JS, Tanoeiro L, Lopes-Oliveira R, Vale FF. Vital JS, et al. Biomolecules. 2022 May 11;12(5):691. doi: 10.3390/biom12050691. Biomolecules. 2022. PMID: 35625618 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical