Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli - PubMed (original) (raw)
Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli
V Weiss et al. Proc Natl Acad Sci U S A. 1988 Dec.
Abstract
It has previously been shown that phosphorylated nitrogen regulator I (NRI-phosphate) is the activator responsible for increasing the transcription of glnA, the structural gene for glutamine synthetase, and that NRII catalyzes the transfer of the gamma-phosphate of ATP to NRI. We have now shown that the reaction of ATP with NRII results in the reversible transfer of the gamma-phosphate of ATP to a histidine residue of NRII. In turn, NRII-phosphate transfers its phosphate reversibly to an aspartic residue of NRI. NRI-phosphate is hydrolyzed to NRI and inorganic phosphate in a divalent cation-requiring autocatalytic reaction.
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