Frequency among Enterobacteriaceae of the DNA sequences encoding type 1 pili - PubMed (original) (raw)

Frequency among Enterobacteriaceae of the DNA sequences encoding type 1 pili

K Buchanan et al. J Bacteriol. 1985 May.

Abstract

Type 1 pili, characterized by mannose-inhibitable agglutination of fowl or guinea pig erythrocytes, have been found throughout the family Enterobacteriaceae. A radiolabeled probe was prepared from a restriction endonuclease-digested fragment of the Escherichia coli pil operon and used to detect homologous DNA sequences in 236 bacteria representing 11 genera of Enterobacteriaceae. Only isolates identified as E. coli or Shigella spp. exhibited homology. In contrast, mannose-sensitive hemagglutination was observed in nine genera. Probe DNA did not hybridize to plasmid DNA, indicating a chromosomal location for the pil operon. Analysis of restriction nuclease-digested whole-cell DNA from 60 E. coli and two Shigella sp. isolates indicated that internal sequences were conserved in most strains, but that changes in flanking sequences in the chromosome were common.

PubMed Disclaimer

References

    1. Trans N Y Acad Sci. 1965 Jun;27(8):1003-54 -PubMed
    1. J Pathol Bacteriol. 1966 Jul;92(1):107-38 -PubMed
    1. J Gen Microbiol. 1968 Apr;51(1):1-16 -PubMed
    1. Biochemistry. 1969 Aug;8(8):3289-95 -PubMed
    1. J Med Microbiol. 1969 Nov 4;2(4):535-53 -PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources