In Vitro Plant Regeneration from Commercial Cultivars of Soybean - PubMed (original) (raw)

In Vitro Plant Regeneration from Commercial Cultivars of Soybean

Ghulam Raza et al. Biomed Res Int. 2017.

Abstract

Soybean, a major legume crop, is the source of vegetable oil and protein. There is a need for transgenic approaches to breeding superior soybean varieties to meet future climate challenges. Efficient plant regeneration is a prerequisite for successful application of genetic transformation technology. Soybean cultivars are classified into different maturity groups based on photoperiod requirements. In this study, nine soybean varieties belonging to different maturity group were regenerated successfully from three different explants: half split hypocotyl, complete hypocotyl, and cotyledonary node. All the genotypes and explant types responded by producing adventitious shoots. Shoot induction potential ranged within 60-87%, 50-100%, and 75-100%, and regeneration rate ranged within 4.2-10, 2.7-4.2, and 2.6-10.5 shoots per explant using half split hypocotyl, complete hypocotyl, and cotyledonary explants, respectively, among all the tested genotypes. Bunya variety showed the best regeneration response using half split and complete hypocotyl explants and the PNR791 with cotyledonary node. The regenerated shoots were successfully rooted and acclimatized to glasshouse conditions. This study shows that commercial varieties of soybean are amenable to shoot regeneration with high regeneration frequencies and could be exploited for genetic transformation. Further, our results show no correlation between shoots regeneration capacity with the maturity grouping of the soybean cultivars tested.

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Figures

Figure 1

Figure 1

Tissue culture steps in development of soybean plants (cv. Bunya as a representative); (a) soybean seeds on germination medium; (b) 6-day-old seedlings; (c, d, e) half split hypocotyl, complete hypocotyl, and cotyledonary node explants on shoot induction medium (day 0); (f, g, h) half split hypocotyls, complete hypocotyls, and cotyledonary explants on shoot induction medium after 15 days; (i, j, k) regenerated shoots from half split hypocotyl, complete hypocotyl, and cotyledonary node explants after 45 days; (l, m, n) shoots from half split hypocotyl, complete hypocotyl, and cotyledonary node explants on rooting medium.

Figure 2

Figure 2

Normal plant growth of tissue culture plants (cv. Bunya as a representative): (a) rooted plant, (b) growth in soil, (c) pods development, and (d) viable pollens.

Figure 3

Figure 3

Cultivars showing maximum and minimum regeneration from three different explants types.

Figure 4

Figure 4

Regeneration response of nine soybean cultivars from three different explants types. Error bars indicate the standard error of mean (n = 3).

Figure 5

Figure 5

Cultivars showing maximum and minimum proliferation after one month of subculturing from three different explants types; (a) half split hypocotyls, (b) complete hypocotyls, and (c) cotyledonary node.

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