Nucleotide sequence of the Azospirillum brasilense Sp7 glutamine synthetase structural gene - PubMed (original) (raw)
Comparative Study
Nucleotide sequence of the Azospirillum brasilense Sp7 glutamine synthetase structural gene
H Bozouklian et al. Biochimie. 1986 Oct-Nov.
Abstract
The complete nucleotide sequence of the glnA gene, encoding the glutamine synthetase subunit of Azospirillum brasilense Sp7, was established. This is the first Azospirillum gene sequenced. The gene encodes a 468 residue polypeptide of MW 51,917. The similarity coefficient (SAB) between the polypeptidic sequence of Azospirillum and Anabaena 7120, which is the only other glnA sequence available, is 58%. No significant homology with E. coli canonical and ntr promoters, or with the promoter region of the Anabaena glnA gene was found. When fused to an E. coli promoter, the gene could be translated in E. coli, despite a very biased codon usage and an atypical Shine-Dalgarno sequence.
Similar articles
- Cloning and characterization of the glnA gene of Azospirillum brasilense Sp7.
Bozouklian H, Fogher C, Elmerich C. Bozouklian H, et al. Ann Inst Pasteur Microbiol (1985). 1986 Jul-Aug;137B(1):3-18. doi: 10.1016/s0769-2609(86)80089-8. Ann Inst Pasteur Microbiol (1985). 1986. PMID: 2893582 - Characterization of three different nitrogen-regulated promoter regions for the expression of glnB and glnA in Azospirillum brasilense.
de Zamaroczy M, Delorme F, Elmerich C. de Zamaroczy M, et al. Mol Gen Genet. 1990 Dec;224(3):421-30. doi: 10.1007/BF00262437. Mol Gen Genet. 1990. PMID: 1702507 - Characterization of the gene encoding glutamine synthetase I (glnA) from Bradyrhizobium japonicum.
Carlson TA, Guerinot ML, Chelm BK. Carlson TA, et al. J Bacteriol. 1985 May;162(2):698-703. doi: 10.1128/jb.162.2.698-703.1985. J Bacteriol. 1985. PMID: 2859270 Free PMC article. - Regulation of Escherichia coli glutamine synthetase.
Rhee SG, Chock PB, Stadtman ER. Rhee SG, et al. Adv Enzymol Relat Areas Mol Biol. 1989;62:37-92. doi: 10.1002/9780470123089.ch2. Adv Enzymol Relat Areas Mol Biol. 1989. PMID: 2567108 Review. No abstract available. - Evolution of glutamine synthetase genes is in accordance with the neutral theory of molecular evolution.
Tateno Y. Tateno Y. Jpn J Genet. 1994 Oct;69(5):489-502. doi: 10.1266/jjg.69.489. Jpn J Genet. 1994. PMID: 7999370 Review.
Cited by
- Phenotypic changes resulting from distinct point mutations in the Azospirillum brasilense glnA gene, encoding glutamine synthetase.
Van Dommelen A, Keijers V, Wollebrants A, Vanderleyden J. Van Dommelen A, et al. Appl Environ Microbiol. 2003 Sep;69(9):5699-701. doi: 10.1128/AEM.69.9.5699-5701.2003. Appl Environ Microbiol. 2003. PMID: 12957965 Free PMC article. - P(II) signal transduction proteins, pivotal players in microbial nitrogen control.
Arcondéguy T, Jack R, Merrick M. Arcondéguy T, et al. Microbiol Mol Biol Rev. 2001 Mar;65(1):80-105. doi: 10.1128/MMBR.65.1.80-105.2001. Microbiol Mol Biol Rev. 2001. PMID: 11238986 Free PMC article. Review. - Novel lysophospholipase A secreted by Legionella pneumophila.
Flieger A, Gong S, Faigle M, Stevanovic S, Cianciotto NP, Neumeister B. Flieger A, et al. J Bacteriol. 2001 Mar;183(6):2121-4. doi: 10.1128/JB.183.6.2121-2124.2001. J Bacteriol. 2001. PMID: 11222614 Free PMC article. - Molecular cloning and sequence analysis of an Azospirillum brasilense indole-3-pyruvate decarboxylase gene.
Costacurta A, Keijers V, Vanderleyden J. Costacurta A, et al. Mol Gen Genet. 1994 May 25;243(4):463-72. doi: 10.1007/BF00280477. Mol Gen Genet. 1994. PMID: 8202090 - Functional organization of the glnB-glnA cluster of Azospirillum brasilense.
de Zamaroczy M, Paquelin A, Elmerich C. de Zamaroczy M, et al. J Bacteriol. 1993 May;175(9):2507-15. doi: 10.1128/jb.175.9.2507-2515.1993. J Bacteriol. 1993. PMID: 8097514 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical