Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxiliary protein - PubMed (original) (raw)

. 1987 Apr;326(6112):517-20.

doi: 10.1038/326517a0.

PMID: 2882424
DOI: 10.1038/326517a0

Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxiliary protein

G Prelich et al. Nature. 1987 Apr.

Abstract

The mechanism of replication of the simian virus 40 (SV40) genome closely resembles that of cellular chromosomes, thereby providing an excellent model system for examining the enzymatic requirements for DNA replication. Only one viral gene product, the large tumour antigen (large-T antigen), is required for viral replication, so the majority of replication enzymes must be cellular. Indeed, a number of enzymatic activities associated with replication and the S phase of the cell cycle are induced upon SV40 infection. Cell-free extracts derived from human cells, when supplemented with immunopurified SV40 large-T antigen support efficient replication of plasmids that contain the SV40 origin of DNA replication. Using this system, a cellular protein of relative molecular mass 36,000 (Mr = 36K) that is required for the elongation stage of SV40 DNA replication in vitro has been purified and identified as a known cell-cycle regulated protein, alternatively called the proliferating cell nuclear antigen (PCNA) or cyclin. It was noticed that, in its physical characteristics, PCNA closely resembles a protein that regulates the activity of calf thymus DNA polymerase-delta. Here we show that PCNA and the polymerase-delta auxiliary protein have similar electrophoretic behaviour and are both recognized by anti-PCNA human autoantibodies. More importantly, both proteins are functionally equivalent; they stimulate SV40 DNA replication in vitro and increase the processivity of calf thymus DNA polymerase-delta. These results implicate a novel animal cell DNA polymerase, DNA polymerase-delta, in the elongation stage of replicative DNA synthesis in vitro.

PubMed Disclaimer

Cited by

Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta.
Yang CL, Chang LS, Zhang P, Hao H, Zhu L, Toomey NL, Lee MY. Yang CL, et al. Nucleic Acids Res. 1992 Feb 25;20(4):735-45. doi: 10.1093/nar/20.4.735. Nucleic Acids Res. 1992. PMID: 1542570 Free PMC article.
H3K4me3 demethylation by the histone demethylase KDM5C/JARID1C promotes DNA replication origin firing.
Rondinelli B, Schwerer H, Antonini E, Gaviraghi M, Lupi A, Frenquelli M, Cittaro D, Segalla S, Lemaitre JM, Tonon G. Rondinelli B, et al. Nucleic Acids Res. 2015 Mar 11;43(5):2560-74. doi: 10.1093/nar/gkv090. Epub 2015 Feb 23. Nucleic Acids Res. 2015. PMID: 25712104 Free PMC article.
An inhibitor of DNA polymerases alpha and delta in calf thymus DNA.
Goulian M, Heard CJ. Goulian M, et al. Nucleic Acids Res. 1990 Aug 25;18(16):4791-6. doi: 10.1093/nar/18.16.4791. Nucleic Acids Res. 1990. PMID: 2118625 Free PMC article.
Molecular cloning and structural analysis of mouse gene and pseudogenes for proliferating cell nuclear antigen.
Yamaguchi M, Hayashi Y, Hirose F, Matsuoka S, Moriuchi T, Shiroishi T, Moriwaki K, Matsukage A. Yamaguchi M, et al. Nucleic Acids Res. 1991 May 11;19(9):2403-10. doi: 10.1093/nar/19.9.2403. Nucleic Acids Res. 1991. PMID: 1674997 Free PMC article.
Review: assessment of cell proliferation in histological material.
Hall PA, Levison DA. Hall PA, et al. J Clin Pathol. 1990 Mar;43(3):184-92. doi: 10.1136/jcp.43.3.184. J Clin Pathol. 1990. PMID: 2185282 Free PMC article. Review. No abstract available.

Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxiliary protein - PubMed (original) (raw)